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Trimodal chromatography enables facile peptide fractionation

ABSTRACT: We evaluated the utility of a trimodal stationary phase (Trinity P1) in offline peptide fractionation, and found it performed similarly to ion exchange or high pH reversed phase based techniques. It is orthogonal to low pH reversed phase and uses MS compatible mobile phase solvents.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Cell Culture

SUBMITTER: Peng Yu

LAB HEAD: Bernhard Kuster

PROVIDER: PXD006188 | Pride | 2017-08-07

REPOSITORIES: Pride

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			Action	DRS
	01703_A01_P018141_S00_N01_R1.raw	Raw
	01703_A02_P018141_S00_N16_R1.raw	Raw
	01703_A03_P018141_S00_N17_R1.raw	Raw
	01703_A04_P018141_S00_N25_R1.raw	Raw
	01703_A05_P018141_S00_N33_R1.raw	Raw

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Publications

Trimodal Mixed Mode Chromatography That Enables Efficient Offline Two-Dimensional Peptide Fractionation for Proteome Analysis.

Yu Peng P Petzoldt Svenja S Wilhelm Mathias M Zolg Daniel Paul DP Zheng Runsheng R Sun Xuefei X Liu Xiaodong X Schneider Günter G Huhmer Andreas A Kuster Bernhard B

Analytical chemistry 20170818 17

Offline two-dimensional chromatography is a common means to achieve deep proteome coverage. To reduce sample complexity and dynamic range and to utilize mass spectrometer (MS) time efficiently, high chromatographic resolution of and good orthogonality between the two dimensions are needed. Ion exchange and high pH reversed phase chromatography are often used for this purpose. However, the former requires desalting to be MS-compatible, and the latter requires fraction pooling to create orthogonal ...[more]

PMID: 28759994

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