Dataset Information

T cells facilitate brain metastasis of breast cancer.

ABSTRACT: Three breast cancer cell lines were co-cultured with T cells isolated from normal donors, in 3: 1 ratio. As controls, the breast cancer cells were cultured without T cells for three days. All cell cultures were washed three times with PBS to remove T cells before they were scraped and collected in 1.5 mL Eppendorf tubes. Three additional washing steps with PBS were applied. After removing the supernatant, the cell pellets were immediately snap-frozen on dry-ice and stored in -80 °C until the time of preparation. After thawing the samples, they were prepared and measured on nano LC.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Dana Mustafa

LAB HEAD: Johan M. Kros

PROVIDER: PXD006750 | Pride | 2018-01-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	150325O2c1_DM-0837-231-Tcell_034.raw	Raw
	150325O2c1_DM-0837-231_033.raw	Raw
	150325O2c1_DM-0837-B2M2-Tcell_036.raw	Raw
	150325O2c1_DM-0837-B2M2_035.raw	Raw
	150325O2c1_DM-0837-SUM-TCRA2_038.raw	Raw

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1 - 5 of 18

Publications

T lymphocytes facilitate brain metastasis of breast cancer by inducing Guanylate-Binding Protein 1 expression.

Mustafa Dana A M DAM Pedrosa Rute M S M RMSM Smid Marcel M van der Weiden Marcel M de Weerd Vanja V Nigg Alex L AL Berrevoets Cor C Zeneyedpour Lona L Priego Neibla N Valiente Manuel M Luider Theo M TM Debets Reno R Martens John W M JWM Foekens John A JA Sieuwerts Anieta M AM Kros Johan M JM

Acta neuropathologica 20180119 4

The discovery of genes and molecular pathways involved in the formation of brain metastasis would direct the development of therapeutic strategies to prevent this deadly complication of cancer. By comparing gene expression profiles of Estrogen Receptor negative (ER-) primary breast tumors between patients who developed metastasis to brain and to organs other than brain, we found that T lymphocytes promote the formation of brain metastases. To functionally test the ability of T cells to promote b ...[more]

PMID: 29350274

Dataset Information

T cells facilitate brain metastasis of breast cancer.

Dataset's files

Publications

T lymphocytes facilitate brain metastasis of breast cancer by inducing Guanylate-Binding Protein 1 expression.

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