Proteomics

Dataset Information

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BPA Gestational Exposure Affects F1 Capacitated Spermatozoa


ABSTRACT: Proteomic investigations of spermatozoa provide practical tools for distinguishing normal, functional spermatozoa from abnormal spermatozoa. Indeed, two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS) applies high-throughput industrial applications to identify sperm-specific proteins indicative of chemical exposure. As such, a direct comparison of protein expression profiles between control and exposed cells returned a set of protein markers. Because mature mammalian spermatozoa are virtually incapable of protein synthesis, the predicted protein biomarkers in spermatozoa offer considerable stability for use in clinical application. In the current study, we applied 2-DE coupled with ESI-MS/MS to investigate the modified protein profile in F1 capacitated spermatozoa due to gestational bisphenol-A (BPA) exposure to ascertain whether these proteomic modifications could explain the observed functional alterations in spermatozoa.

INSTRUMENT(S): micrOTOF-Q III

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spermatozoon, Sperm

DISEASE(S): Disease Free

SUBMITTER: Md Saidur Rahman  

LAB HEAD: Myung-Geol Pang

PROVIDER: PXD006829 | Pride | 2018-01-02

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
AC1112.mgf Mgf
AC2207.mgf Mgf
AC2421.mgf Mgf
AC2423.mgf Mgf
AC3215_663.mgf Mgf
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Publications

Functional and Proteomic Alterations of F1 Capacitated Spermatozoa of Adult Mice Following Gestational Exposure to Bisphenol A.

Rahman Md Saidur MS   Kwon Woo-Sung WS   Ryu Do-Yeal DY   Khatun Amena A   Karmakar Polash Chandra PC   Ryu Buom-Yong BY   Pang Myung-Geol MG  

Journal of proteome research 20171221 1


Studies regarding bisphenol A (BPA) exposure and male (in)fertility have conventionally focused on modifications in ejaculated spermatozoa function from exposed individuals. However, mammalian spermatozoa are incapable of fertilization prior to achieving capacitation, the penultimate step in maturation. Therefore, it is necessary to investigate BPA-induced changes in capacitated spermatozoa and assess the consequences on subsequent fertilization. Here, we demonstrate the effect of gestational BP  ...[more]

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