Dataset Information
Nanodroplet processing platform for deep, quantitative proteome profiling of 10-100 mammalian cells
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Pancreatic Islet
SUBMITTER: 
Matthew Monroe
LAB HEAD: Ryan T. Kelly
PROVIDER: PXD006847 | Pride | 2018-01-24
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| CulturedCells_parameters.txt | Txt | |||
| CulturedCells_peptides.txt | Txt | |||
| CulturedCells_proteinGroups.txt | Txt | |||
| CulturedCells_summary.txt | Txt | |||
| Islet_t1d_ct_parameters.txt | Txt |
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Publications
Nanodroplet processing platform for deep and quantitative proteome profiling of 10-100 mammalian cells.
Nature communications 20180228 1
Nanoscale or single-cell technologies are critical for biomedical applications. However, current mass spectrometry (MS)-based proteomic approaches require samples comprising a minimum of thousands of cells to provide in-depth profiling. Here, we report the development of a nanoPOTS (nanodroplet processing in one pot for trace samples) platform for small cell population proteomics analysis. NanoPOTS enhances the efficiency and recovery of sample processing by downscaling processing volumes to <20 ...[more]
