Molecular effects of Vitamin C treatment in MSC80 cells, a suitable Schwann cell model

ABSTRACT: Schwann cells (SCs) are not only decisive to produce the axon-wrapping myelin sheath thus ensuring a proper nerve conduction but also exhibit with trophic function and can direct repair mechanisms of the peripheral nervous system. Consequently, suitable and well-characterized SC in vitro models are needed to perform appropriate pre-clinical studies including the investigation of the complex biochemical adaptations occurring in the peripheral nervous system (PNS) under different (patho)physiological conditions. MSC80 cells represent a SC line derived from mouse used in laboratories as a suitable in vitro system for neuropathological studies. As a comprehensive MSC80 protein catalogue remained elusive, here we introduce the most abundant 9532 proteins identified via mass spectrometry based protein analytics and thus provide the most comprehensive SC protein catalogue published thus far. Hereby, not only proteins causative for inherited neuropathies were covered but it has also been demonstrated that apart from cytoplasmic and nuclear proteins, mitochondrial proteins and such belonging to the protein processing machinery are very well covered. Moreover, we addressed the suitability of MSC80 to examine molecular effect of a drug-treatment by analyzing the proteomic signature of Vitamin C-treated MSC80 cells. Proteomic findings along with further immunological and functional experiments support the concept of a beneficial role influence of Vitamin C on oxidative stress and identified TMX1 as an oxidative stress protective factor in SC which might represent a promising target for therapeutic intervention of PNS disorders with marked oxidative stress burden such as diabetic neuropathy.

INSTRUMENT(S): Orbitrap Fusion Lumos, LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Vietxuan Phan  

LAB HEAD: Andreas Roos

PROVIDER: PXD006910 | Pride | 2018-08-13

REPOSITORIES: Pride