Proteomics

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He Xiang - BfrB - Systematic Identification of Mycobacterium tuberculosis Effectors Reveals that BfrB Suppresses Innate Immunity

ABSTRACT: To identify the macrophage binding partner of Mtb BfrB, we selected and purified BfrB tagged with GST, and incubated it with THP-1 lysates before performing GST Pullodwn. Then the eluted protein were examined by sliver stain. Using mass spectrometry, we identified a protein with an apparent molecular mass of 28 KDa as RPS3, an essential subunit of NF-κB involved in the regulation of key genes in rapid cellular activation response.

INSTRUMENT(S): ultraflex

ORGANISM(S): Homo Sapiens (human) Mycobacterium Tuberculosis

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: He Xiang  

LAB HEAD: Tao Shengce

PROVIDER: PXD006929 | Pride | 2017-10-17

REPOSITORIES: Pride

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20140320_HX_1_GB6_01_3758.d.mzid.gz Mzid
20140320_HX_1_GB6_01_3758.d.pride.mztab.gz Mztab
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Systematic Identification of <i>Mycobacterium tuberculosis</i> Effectors Reveals that BfrB Suppresses Innate Immunity.

He Xiang X   Jiang He-Wei HW   Chen Hong H   Zhang Hai-Nan HN   Liu Yin Y   Xu Zhao-Wei ZW   Wu Fan-Lin FL   Guo Shu-Juan SJ   Hou Jing-Li JL   Yang Ming-Kun MK   Yan Wei W   Deng Jiao-Yu JY   Bi Li-Jun LJ   Zhang Xian-En XE   Tao Sheng-Ce SC  

Molecular & cellular proteomics : MCP 20171010 12

<i>Mycobacterium tuberculosis</i> (Mtb) has evolved multiple strategies to counter the human immune system. The effectors of Mtb play important roles in the interactions with the host. However, because of the lack of highly efficient strategies, there are only a handful of known Mtb effectors, thus hampering our understanding of Mtb pathogenesis. In this study, we probed Mtb proteome microarray with biotinylated whole-cell lysates of human macrophages, identifying 26 Mtb membrane proteins and se  ...[more]

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