Dataset Information

Quantitative proteomics of barrier-deficient mouse epidermis in response to environmental humidity

ABSTRACT: Low environmental humidity aggravates symptoms of inflammatory skin diseases, e.g. of Atopic Dermatitis (AD). Using mice that develop AD-like symptoms, we show that an increase in environmental humidity rapidly rescues their cutaneous inflammation and associated epidermal abnormalities. Quantitative proteomics analysis of epidermal lysates of mice kept at low or high humidity identified novel humidity-regulated proteins, including Clca2/Clca3a2, a protein with previously unknown function in the skin.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Skin

SUBMITTER: Ulrich auf dem Keller

LAB HEAD: Sabine Werner

PROVIDER: PXD007124 | Pride | 2018-05-14

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20140620_FGFRxHumidity_GoldenLane_30percent_coisolation.pdResult	Other
	20140620_FGFRxHumidity_GoldenLane_30percent_coisolation.pdResultView	Other
	20140620_humid_fract_setA_03_B2.raw	Raw
	20140620_humid_fract_setA_04_B3.raw	Raw
	20140620_humid_fract_setA_05_B4.raw	Raw

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Publications

Humidity-regulated CLCA2 protects the epidermis from hyperosmotic stress.

Seltmann Kristin K Meyer Michael M Sulcova Jitka J Kockmann Tobias T Wehkamp Ulrike U Weidinger Stephan S Auf dem Keller Ulrich U Werner Sabine S

Science translational medicine 20180501 440

Low environmental humidity aggravates symptoms of the inflammatory skin disease atopic dermatitis (AD). Using mice that develop AD-like signs, we show that an increase in environmental humidity rescues their cutaneous inflammation and associated epidermal abnormalities. Quantitative proteomics analysis of epidermal lysates of mice kept at low or high humidity identified humidity-regulated proteins, including chloride channel accessory 3A2 (CLCA3A2), a protein with previously unknown function in ...[more]

PMID: 29743348

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