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SWATH-MS proteomic analysis of nitrogen deficiency response of Arabidopsis

ABSTRACT: Protein profiling of Arabidopsis thaliana seedling under nitrogen depreciation. Seven days old seedlings of Aradidopsis thaliana were transferred to nitrogen depreciated MS medium for further 48 hours cultivation. Same number of seedlings received same treatment except using MS medium were acted as control. After treatment, whole seedlings were extracted for protein profiling study.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell

DISEASE(S): Disease Free

SUBMITTER: Wai Lung Chan

LAB HEAD: Wai-Lung Chan

PROVIDER: PXD007190 | Pride | 2018-11-21

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Lib6-9.group	Other
	SWATHC3.wiff	Wiff
	SWATHC3.wiff.scan	Wiff
	SWATHC4.wiff	Wiff
	SWATHC4.wiff.scan	Wiff

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1 - 5 of 29

Publications

SWATH-MS quantitative proteomic investigation of nitrogen starvation in Arabidopsis reveals new aspects of plant nitrogen stress responses.

Zhu Fu-Yuan FY Chen Mo-Xian MX Chan Wai-Lung WL Yang Feng F Tian Yuan Y Song Tao T Xie Li-Juan LJ Zhou Ying Y Xiao Shi S Zhang Jianhua J Lo Clive C

Journal of proteomics 20180723

Nitrogen is an essential macronutrient for plant growth and crop productivity. The aim of this work was to further investigate the molecular events during plant adaptation to nitrogen stress. Here, we present a SWATH-MS (Sequential window acquisition of all theoretical mass spectra)-based quantitative approach to detect proteome changes in Arabidopsis seedlings following nitrogen starvation. In total, 736 proteins of diverse functions were determined to show significant abundance changes between ...[more]

PMID: 30048775

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Expression profile of Arabidopsis thaliana seedlings subjected to hypoxia

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Transcription profiling by array of Brachypodium response to increased ambient temperature in seedlings

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