Dataset Information

Porcine LC-MSMS - Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture

ABSTRACT: Porcine breast fatty extracellular matrix proetiens has been extracted after decellularization of fatty tissue with conventional method (PFT-1) using triton X-100 and sodium dodecyl sulfate and extracted with modified methods keeping natural protein in their natural condition using X-100, urea, thiourea etc

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Skin

SUBMITTER: Weimin Li

LAB HEAD: Weimin Li

PROVIDER: PXD011011 | Pride | 2019-11-12

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PFT-1_tissue.raw	Raw
	PFT-1_tissueF016328.mzid	Mzid
	PFT-1_tissueF016328.mzid_PFT-1_tissue_F016328.MGF	Mzid
	PFT-2_ECM.raw	Raw
	PFT-2_ECMF016323.mzid	Mzid

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Publications

Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture.

Rijal Girdhari G Wang Jing J Yu Ilhan I Gang David R DR Chen Roland K RK Li Weimin W

International journal of molecular sciences 20180925 10

Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expr ...[more]

PMID: 30257480

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