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Moss Physcomitrella paten endogenous peptides, LC-MS/MS, part 2

ABSTRACT: Short (<100 codons) open reading frames (sORFs) are common features of all genomes. Proof of translation of sORFs is an important step towards finding of functional sORF-encoded peptides (SEPs). We verified translation of our predicted sORFs using mass-spectrometry (MS) analysis which is often considered as the gold standard in detection of proteins or peptides in cell. Taking into account the shortage of proteomic methods in identifying small proteins or peptides, in our study we used “peptidomic” dataset - endogenous peptides, extracted from three types of moss cells – gametophores, protonemata and protoplasts

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Physcomitrella Patens Subsp. Patens (moss)

TISSUE(S): Plant Cell, Protonema, Gametophore

SUBMITTER: Igor Fesenko

LAB HEAD: Vadim M Govorun

PROVIDER: PXD007923 | Pride | 2019-08-08

REPOSITORIES: Pride

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			Action	DRS
	gametophores_raw_data.zip	Other
	gametophores_search.zip	Other
	protonema_raw_data.zip	Other
	protonema_search.zip	Other
	protoplasts_raw_data.zip	Other

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Moss endogenous peptides, LC-MS/MS

Project description:Short (<100 codons) open reading frames (sORFs) are common features of all genomes. Proof of translation of sORFs is an important step towards finding of functional sORF-encoded peptides (SEPs). We verified translation of our predicted sORFs using mass-spectrometry (MS) analysis which is often considered as the gold standard in detection of proteins or peptides in cell. Taking into account the shortage of proteomic methods in identifying small proteins or peptides, in our study we used “peptidomic” dataset - endogenous peptides, extracted from three types of moss cells – gametophores, protonemata and protoplasts

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