Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Xenopus Laevis (african Clawed Frog)
TISSUE(S): Egg
SUBMITTER: Mario Oroshi
LAB HEAD: Matthias Mann
PROVIDER: PXD008831 | Pride | 2019-02-13
REPOSITORIES: Pride
Items per page: 1 - 5 of 54 |
Molecular cell 20181227 3
DNA-protein crosslinks (DPCs) are bulky lesions that interfere with DNA metabolism and therefore threaten genomic integrity. Recent studies implicate the metalloprotease SPRTN in S phase removal of DPCs, but how SPRTN is targeted to DPCs during DNA replication is unknown. Using Xenopus egg extracts that recapitulate replication-coupled DPC proteolysis, we show that DPCs can be degraded by SPRTN or the proteasome, which act as independent DPC proteases. Proteasome recruitment requires DPC polyubi ...[more]