Dataset Information

Pneumococcal surface tryptic digestion

ABSTRACT: Surface digestion of living pneumococcal cells grown in different media that attempt to simulate different infection models (for example, in culture media containing the soluble secreted fraction orignated by the growth of macrophages and epithelial cells)

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Streptococcus Pneumoniae

TISSUE(S): Prokaryotic Cell

DISEASE(S): Pleural Empyema

SUBMITTER: Alfonso Olaya Abril

LAB HEAD: Manuel José Rodríguez Ortega

PROVIDER: PXD008885 | Pride | 2022-02-15

REPOSITORIES: Pride

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Publications

Approaching In Vivo Models of Pneumococcus-Host Interaction: Insights into Surface Proteins, Capsule Production, and Extracellular Vesicles.

Olaya-Abril Alfonso A González-Reyes José A JA Rodríguez-Ortega Manuel J MJ

Pathogens (Basel, Switzerland) 20210828 9

Infections caused by the Gram-positive bacterium <i>Streptococcus pneumoniae</i> have become a major health problem worldwide because of their high morbidity and mortality rates, especially in developing countries. This microorganism colonizes the human upper respiratory tract and becomes pathogenic under certain circumstances, which are not well known. In the interaction with the host, bacterial surface structures and proteins play major roles. To gain knowledge into gradual changes and adaptiv ...[more]

PMID: 34578131

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Project description:Influenza A virus (IAV) predisposes individuals to secondary infections with the bacterium Streptococcus pneumoniae (the pneumococcus). Infections may manifest as pneumonia, sepsis, meningitis or otitis media (OM). It remains controversial as to whether secondary pneumococcal disease is due to the induction of an aberrant immune response or IAV induced immunosuppression. Moreover, as the majority of studies have been performed in the context of pneumococcal pneumonia, it remains unclear how far these findings can be extrapolated to other pneumococcal disease phenotypes. Here, we demonstrate that the viral hemagglutinin (HA) mediates bacterial OM by inducing a pro-inflammatory response in the middle ear cavity in a replication-dependent manner. Importantly, our findings show that it is the inflammatory response that mediates pneumococcal replication; not viral suppression of the immune system or epithelial damage. This study provide the first evidence that HA induced inflammation drives pneumococcal replication in the middle ear cavity, which has important consequences to the treatment of pneumococcal OM. Five-day old C57BL/6 mice were colonised intranasally (i.n.) with 2M-CM-^W103 colony forming units (CFU) of S. pneumoniae EF3030Lux in 3 M-BM-5Ls of PBS. Alternatively, mice were mock-infected with an equivalent volume of phosphate buffered saline (PBS). At 14-days of age, infant mice were infected i.n. with 20 plaque forming units (PFU) (PR8/34, Cambridge/34 and WSN/33) or 102.5 PFU (all other virus strains) of egg-grown IAV in 3 M-BM-5Ls of PBS. Viral doses were selected to ensure a reproducible infection with minimum morbidity and no mortality. Six days post-IAV infection, mice were euthanised and organs were collected for analysis. Six independent biological replicates (where both ears from one mouse were pooled to create one sample) were used for each condition and analyzed using the NimbleGen platform (12M-CM-^W135K Mouse Gene Expression Arrays, Roche Nimblegen, USA). Indirect labeling, hybridization and washing was performed according to the manufacturerM-bM-^@M-^Ys instructions. Array images were acquired with a NimbleGen MS200 scanner, and images were processed with NimbleScan software using the RMA algorithm. Data was processed using Arraystar (DNASTAR, USA) with default settings as described in the manual. Differential expression tests were performed with a moderated T-test implemented in Arraystar, followed by FDR correction of the P values (Q-values) according to the method of Storey and Tibshirani

			Action	DRS
	NewStudy.pdStudy	Other
	NewStudy.pdStudy.bak	Other
	NewStudy.pdStudy.temp.bak	Other
	R6_DMEM.msf	Msf
	R6_DMEM.msfView	Msf