Dataset Information

FlashPack: Fast and simple preparation of ultra-high performance capillary columns for LC-MS

ABSTRACT: High-resolution capillary chromatography is essential for in-depth characterization of complex biomolecule mixtures by LC-MS. We report a simple and low-cost FlashPack protocol for preparation of ultra-high performance capillary columns of > 50 cm length in less than 1 h using low-pressure equipment. Flashpack LC columns enable robust identification of >35,000 unique peptides and 5,800 human proteins in a single LC-MS analysis.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Myotube, Fibroblast

SUBMITTER: Adelina Rogowska-Wrzesinska

LAB HEAD: Adelina Rogowska-Wrzesinska

PROVIDER: PXD009519 | Pride | 2019-01-21

REPOSITORIES: Pride

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			Action	DRS
	Extra_LUM_02485_KS_HeLa_Vlad_1ug_col3_EC50In_250nlMin_1hG.raw	Raw
	Extra_LUM_02486_KS_HeLa_Vlad_1ug_col3_EC50In_250nlMin_4hG.raw	Raw
	Fig2_LUM_02447_KS_HeLa_FASP_3ug_col3_200nlMin_2hG.raw	Raw
	Fig2_LUM_02451_KS_HeLa_FASP_3ug_easySpr_200nlMin_2hG.raw	Raw
	Fig2_LUM_02483_KS_HeLa_Vlad_1ug_easySpr_250nlMin_2hG.raw	Raw

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FlashPack: Fast and Simple Preparation of Ultrahigh-performance Capillary Columns for LC-MS.

Kovalchuk Sergey I SI Jensen Ole N ON Rogowska-Wrzesinska Adelina A

Molecular & cellular proteomics : MCP 20181029 2

Capillary ultrahigh-pressure liquid chromatography (cUHPLC) is essential for in-depth characterization of complex biomolecule mixtures by LC-MS. We developed a simple and fast method called <i>FlashPack</i> for custom packing of capillary columns of 50-100 cm length with sub- 2 μm sorbent particles. <i>FlashPack</i> uses high sorbent concentrations of 500-1,000 mg/ml for packing at relatively low pressure of 100 bar. Column blocking by sorbent aggregation is avoided during the packing by gentle ...[more]

PMID: 30373789

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