Proteomics

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Impact of Cd-exposure on medicago sativa cell wall


ABSTRACT: The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences. It is unessential and harmful to all life forms. The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure. In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd. Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition. Compared to most studies the plants were exposed to the heavy metal for an entire season, including the repeated cutting of the above-ground biomass as is done in agriculture.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Medicago Sativa (alfalfa)

TISSUE(S): Plant Cell, Shoot

SUBMITTER: kjell sergeant  

LAB HEAD: Jean-François Hausman

PROVIDER: PXD009670 | Pride | 2019-06-11

REPOSITORIES: Pride

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Publications

Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?

Gutsch Annelie A   Sergeant Kjell K   Keunen Els E   Prinsen Els E   Guerriero Gea G   Renaut Jenny J   Hausman Jean-Francois JF   Cuypers Ann A  

BMC plant biology 20190621 1


<h4>Background</h4>The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences. It is unessential and harmful to all life forms. The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure. In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd. Liquid chromatography coupled to mass spe  ...[more]

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