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19Jan2018_Suping_LCM_TomatoHeatPollen_protein only

ABSTRACT: Heat stress is a major challenge for crop production. During the reproduction stage, pollen development is the most sensitive process to abnormal environmental conditions. In this project, we have developed a heat treatment system for tomato where plants produce a significantly lower number of pollens and the germination rate of those pollens was also very low. The size of the flower buds and pollen developmental stages were examined under confocal microscope. Pollen cells at different developmental stages were collected from flower buds at corresponding sizes using LCM. The single cell population proteomics analysis was used to identify proteome changes in a distinct group of cells. This information was used to determine the function of proteins that are associated with heat stress on cellular processes/functions during pollen development.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Bemisia Sp. Unknown_th_13_12_s.lycopersicum

TISSUE(S): Plant Cell, Pollen

SUBMITTER: Ted Thannhauser

LAB HEAD: Theodore W. Thannhauser

PROVIDER: PXD010218 | Pride | 2022-02-23

REPOSITORIES: Pride

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			Action	DRS
	19Jan2018_Suping_LCM_Pollen.msf	Msf
	19Jan2018_Suping_LCM_Pollen.mzML	Mzml
	19Jan2018_Suping_LCM_Pollen.mzid	Mzid
	19Jan2018_Suping_LCM_Pollen_F1.raw	Raw
	19Jan2018_Suping_LCM_Pollen_F2.raw	Raw

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Project description:Papaya (Carica papaya) is a trioecious species, with female, male, and hermaphrodite plants. Because of sex segregation, selecting hermaphroditic plants is vital for orchard establishment due to their higher commercial value. In addition to the costly sexing step, environmental stresses can result in abnormal flower development. However, molecular mechanisms that regulate sex differentiation in papaya are still poorly known. Thus, this study aimed to identify proteins associated with sex development in female and hermaphrodite flowers of papaya through comparative proteomic analysis. Proteins from flower buds at the early and late developmental stages of three papaya genotypes (UENF-CALIMAN 01, JS12, and Sunrise Solo 72/12) were studied via proteomic analysis via the combination of the shotgun method and nanoESI-HDMSE technology. In buds at an early stage of development, 496 proteins exhibited significantly different abundances between sexes for the SS72/12 genotype, 139 for the JS12 genotype, and 165 for the UC-01 genotype. At the final stage of development, there were 181 for SS72/12, 113 for JS12, and 125 for UC-01. The large group of differentially accumulated proteins (DAPs) between the sexes was related to metabolism, as shown by the observation of only the proteins that exhibited the same pattern of accumulation in the three genotypes. Specifically, carbohydrate metabolism proteins were up-regulated in hermaphrodite flower buds early in development, while those linked to monosaccharide and amino acid metabolism increased during late development. Enrichment of sporopollenin and phenylpropanoid biosynthesis pathways characterizes hermaphrodite samples across developmental stages, with predicted protein interactions highlighting the crucial role of phenylpropanoids in sporopollenin biosynthesis for pollen wall formation. Most of the DAPs played key roles in pectin, cellulose, and lignin synthesis and were essential for cell wall formation and male flower structure development, notably in the pollen coat. These findings suggest that hermaphrodite flowers require more energy for development, likely due to complex pollen wall formation. Overall, these insights illuminate the molecular mechanisms of papaya floral development, revealing complex regulatory networks and energetic demands in the formation of male reproductive structures.

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19Jan2018_Suping_LCM_TomatoHeatPollen_protein only

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