Dataset Information

Quantitative proteomic analysis of primary prostate cancer tissue specimens

ABSTRACT: Currently, comprehensive and quantitative proteomic analysis of human prostate cancer tissue specimens remains scarce, hindering the identification of protein complexes and pathways deregulated in prostate cancer. In this study, we applied TMT-SPS-MS3-based quantitative proteomics to analzye 9 normal controls, 9 low-grade prostate cancer, and 9 high-grade prostate cancer. About 3,600 proteins were quantified across all the 27 prostate specimens. Statistical analysis identified 651 proteins that are differentially expressed in high-grade prostate cancer and normal prostate. Pathway enrichment analysis revealed that the LXR/RXR activation and integrin signaling pathways are substantially downregulated in high-grade prostate cancer, compared with normal prostate cancer. In addition, protein complex analysis suggested that mitochondrial ribosomes and ribosome-biogenesis complexes are significnatly overexpressed, whereas the cholesterol effluex and focal adhesion comlexes are significantly downregulated in high-grade prostate cancer, compared with normal controls. Furthermore, differential correlation analysis indicated that the spliceosome machinery might be more active in low-grade prostate cancer, compared with normal controls. The results are expected to shed light on the molecular mechnanisms underlying the development and progression of primary prostate cancer in human patients.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Prostate Gland

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: Wei Yang

LAB HEAD: Wei Yang

PROVIDER: PXD010744 | Pride | 2019-04-23

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	Human20180330.fasta	Fasta
	Human20180330_additional.fasta	Fasta
	MaxQuant_Output.zip	Other
	ProstateTMT10plex_S1Fr01.raw	Raw
	ProstateTMT10plex_S1Fr02.raw	Raw

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Publications

Quantitative proteomic analysis of prostate tissue specimens identifies deregulated protein complexes in primary prostate cancer.

Zhou Bo B Yan Yiwu Y Wang Yang Y You Sungyong S Freeman Michael R MR Yang Wei W

Clinical proteomics 20190413

<h4>Background</h4>Prostate cancer (PCa) is the most frequently diagnosed non-skin cancer and a leading cause of mortality among males in developed countries. However, our understanding of the global changes of protein complexes within PCa tissue specimens remains very limited, although it has been well recognized that protein complexes carry out essentially all major processes in living organisms and that their deregulation drives the pathogenesis and progression of various diseases.<h4>Methods ...[more]

PMID: 31011308

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