Dataset Information

Quantitative Analysis of Sheep Muscle Protein (iTRAQ) from College of Animal Science and Technology, China Agricultural University

ABSTRACT: Public description (Provide a description of the study goals and relevance): Adding the effect of exogenous antioxidants on the protein of lamb meat during storage.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Ovis Aries

TISSUE(S): Muscle

SUBMITTER: Chenchen Xu

LAB HEAD: Chenchen Xu

PROVIDER: PXD011174 | Pride | 2021-09-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	All_merged_sub_1.dat-report.xml	Xml
	All_merged_sub_2.dat-report.xml	Xml
	All_merged_sub_3.dat-report.xml	Xml
	All_merged_sub_4.dat-report.xml	Xml
	mzML1.zip	Other

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Publications

The Effect of Dietary Lycopene Supplementation on Drip Loss during Storage of Lamb Meat by iTRAQ Analysis.

Wang Bo B Xu Chen-Chen CC Liu Ce C Qu Yang-Hua YH Zhang Hao H Luo Hai-Ling HL

Antioxidants (Basel, Switzerland) 20210129 2

This study was designed to investigate the impact of dietary lycopene (antioxidant extracted from tomato) supplementation on postmortem antioxidant capacity, drip loss and protein expression profiles of lamb meat during storage. Thirty male Hu lambs were randomly divided into three treatment groups and housed in individual pens and received 0, 200 or 400 mg·kg<sup>-1</sup> lycopene in their diet, respectively. All lambs were slaughtered after 3 months of fattening, and the longissimus thoracis ( ...[more]

PMID: 33573002

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Project description:BackgroundThere are abundant sheep breed resources in the Xinjiang region of China attributing to its diverse ecological system, which include several high-litter size sheep populations. Previous studies have confirmed that the major high prolificacy gene cannot be used to detect high litter size. Our research team found a resource group in Pishan County, southern Xinjiang. It showed high fertility with an average litter size of two to four in one birth, excellent breast development, and a high survival rate of lambs. In the present study, we used this resource as an ideal sample for studying the genetic mechanisms of high prolificacy in sheep.MethodsIndigenous sheep populations from Xinjiang, with different litter sizes, were selected for the research, and specific-locus amplified fragment sequencing (SLAF-seq) technology was used to comprehensively screen single nucleotide polymorphisms (SNPs) from the whole genome that may cause differences in litter size. Novel genes associated with litter size of sheep were detected using genome-wide association studies (GWAS), providing new clues revealing the regulation mechanism of sheep fecundity. Candidate genes related to ovulation and litter size were selected for verification using Kompetitive Allele Specific polymerase chain reaction (KASP) cluster analysis.ResultsWe identified 685,300 SNPs using the SLAF-seq technique for subsequent genome-wide analysis. Subsequently, 155 SNPs were detected at the genome-wide level. Fourteen genes related to sheep reproduction were notated: COIL, SLK, FSHR, Plxna3, Ddx24, CXCL12, Pla2g7, ATP5F1A, KERA, GUCY1A1, LOC101107541, LOC101107119, LOC101107809, and BRAF. Based on literature reports, 30 loci of seven genes and candidate genes (CXCL12, FSHR, SLK, GUCY1A1, COIL, LOC101107541, and LOC101107119) related to ovulation and litter size were selected for verification using KASP cluster analysis. Among them, nine loci of three genes were successfully genotyped. Three loci of FSHR (GenBank ID: 443299, g. 75320741G>A site), GUCY1A1 (GenBank ID: 101110000, g. 43266624C>T site), and COIL (GenBank ID: 101123134, g. 7321466C>G site) were found to be significantly or extremely significantly associated with litter size. These three loci are expected to be used as molecular markers to determine differences in litter size in sheep.