Dataset Information

Global Enrichment of Low-Abundance Peptide Hormones in Plasma for Discovery and Quantitation

ABSTRACT: We describe a method for global enrichment of active peptide hormones from blood plasma for both new hormone discovery and quantitative analysis. To extend the dynamic range of plasma proteomics, we employed high-resolution size exclusion chromatography to purify the peptide hormones and a powerful precipitation method to dissociate and remove bulk proteins prior to separation. Combining this method with high-sensitivity proteomic analysis, we characterized hormone abundance changes after intermittent fasting in humans.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Mark Larance

LAB HEAD: Mark Colin Larance

PROVIDER: PXD011797 | Pride | 2019-07-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20180702_QEHF_ML_DH_EXP124_0pmoles_PRM_REP1.raw	Raw
	20180702_QEHF_ML_DH_EXP124_0pmoles_PRM_REP2.raw	Raw
	20180702_QEHF_ML_DH_EXP124_0pmoles_PRM_REP3.raw	Raw
	20180702_QEHF_ML_DH_EXP124_10pmoles_PRM_REP1.raw	Raw
	20180702_QEHF_ML_DH_EXP124_10pmoles_PRM_REP2.raw	Raw

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Publications

Small-protein Enrichment Assay Enables the Rapid, Unbiased Analysis of Over 100 Low Abundance Factors from Human Plasma.

Harney Dylan J DJ Hutchison Amy T AT Su Zhiduan Z Hatchwell Luke L Heilbronn Leonie K LK Hocking Samantha S James David E DE Larance Mark M

Molecular & cellular proteomics : MCP 20190715 9

Unbiased and sensitive quantification of low abundance small proteins in human plasma (<i>e.g.</i> hormones, immune factors, metabolic regulators) remains an unmet need. These small protein factors are typically analyzed individually and using antibodies that can lack specificity. Mass spectrometry (MS)-based proteomics has the potential to address these problems, however the analysis of plasma by MS is plagued by the extremely large dynamic range of this body fluid, with protein abundances span ...[more]

PMID: 31308252

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