Dataset Information

Secretomic analyses of Ruminiclostridium papyrosolvens reveal its enzymatic basis for lignocellulose degradation

ABSTRACT: Efficient biotechnological conversion of lignocellulosic biomass to valuable products, such as transportation biofuels, is ecologically attractive, yet requires substantially improved mechanistic understanding and optimization to become economically feasible. Cellulolytic clostridia, such as Ruminiclostridium papyrosolvens (previously Clostridium papyrosolvens), produce a wide variety of CAZymes including extracellular multi-enzyme complexes-cellulosomes with different specificities for enhanced cellulosic biomass degradation. Identification of the secretory components, especially CAZymes, during bacterial growth on lignocellulose and their influence on its catalytic capabilities provide insight into construction of potent cellulase systems of cell factories tuned or optimized for the targeted substrate by matching the type and abundance of enzymes and corresponding transporters.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): [clostridium] Papyrosolvens Dsm 2782

SUBMITTER: Mohamed El Hajjami

LAB HEAD: You Wuxin

PROVIDER: PXD013253 | Pride | 2019-11-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MaxQuant_Output.zip	Other
	RP_DSM2782_Cellobiose1_XII.raw	Raw
	RP_DSM2782_Cellobiose2_XII.raw	Raw
	RP_DSM2782_Cellobiose3_XII.raw	Raw
	RP_DSM2782_Cellulose1_X.raw	Raw

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Publications

Secretomic analyses of <i>Ruminiclostridium papyrosolvens</i> reveal its enzymatic basis for lignocellulose degradation.

Ren Zhenxing Z You Wuxin W Wu Shasha S Poetsch Ansgar A Xu Chenggang C

Biotechnology for biofuels 20190715

<h4>Background</h4>Efficient biotechnological conversion of lignocellulosic biomass to valuable products, such as transportation biofuels, is ecologically attractive, yet requires substantially improved mechanistic understanding and optimization to become economically feasible. Cellulolytic clostridia, such as <i>Ruminiclostridium papyrosolvens</i> (previously <i>Clostridium papyrosolvens</i>), produce a wide variety of carbohydrate-active enzymes (CAZymes) including extracellular multienzyme co ...[more]

PMID: 31338125

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Project description:The search for new enzymes and microbial strains to degrade plant biomass is one of the most important strategies for improving the conversion processes in the production of environment-friendly chemicals and biofuels. In this study, we report a new Paenibacillus isolate, O199, which showed the highest efficiency for cellulose deconstruction in a screen of environmental isolates. Here, we provide a detailed description of the complex multi-component O199 enzymatic system involved in the degradation of lignocellulose. We examined the genome and the proteome of O199 grown on complex lignocellulose (wheat straw) and on microcrystalline cellulose. The genome contained 476 genes with domains assigned to carbohydrate-active enzyme (CAZyme) families, including 100 genes coding for glycosyl hydrolases (GHs) putatively involved in cellulose and hemicellulose degradation. Moreover, 31% of these CAZymes were expressed on cellulose and 29% on wheat straw. Proteomic analyses also revealed a complex and complete set of enzymes for deconstruction of cellulose (at least 22 proteins, including 4 endocellulases, 2 exocellulases, 2 cellobiohydrolases and 2 -glucosidases) and hemicellulose (at least 28 proteins, including 5 endoxylanases, 1 -xylosidase, 2 xyloglucanases, 2 endomannanases, 2 licheninases and 1 endo--1,3(4)-glucanase). Most of these proteins were secreted extracellularly and had numerous carbohydrate-binding domains (CBMs). In addition, O199 also secreted a high number of substrate-binding proteins (SBPs), including at least 42 proteins binding carbohydrates. Interestingly, both plant lignocellulose and crystalline cellulose triggered the production of a wide array of hydrolytic proteins, including cellulases, hemicellulases and other GHs. Our data provide an in-depth analysis of the complex and complete set of enzymes and accessory non-catalytic proteins—GHs, CBMs, transporters, and SBPs—implicated in the high cellulolytic capacity shown by this bacterial strain. The large diversity of hydrolytic enzymes and the extracellular secretion of most of them supports the use of Paenibacillus O199 as a candidate for second-generation technologies using paper or lignocellulosic agricultural wastes.