Dataset Information

Protein S-sulfenylation Arabidopsis - AtMAPK4

ABSTRACT: Study the oxidation-sensitivity of recombinant AtMAPK4 by treatment with 0 mM hydrogen peroxide (H2O2) (sample '_01'), 1 mM (sample '_02'), and 10 mM (sample '_03'). Each sample subdivided for trypsin and chymotrypsin digestion.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Patrick Willems

LAB HEAD: Joris Messens

PROVIDER: PXD013588 | Pride | 2019-09-16

REPOSITORIES: Pride

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Publications

Mining for protein S-sulfenylation in Arabidopsis uncovers redox-sensitive sites.

Huang Jingjing J Willems Patrick P Wei Bo B Tian Caiping C Ferreira Renan B RB Bodra Nandita N Martínez Gache Santiago Agustín SA Wahni Khadija K Liu Keke K Vertommen Didier D Gevaert Kris K Carroll Kate S KS Van Montagu Marc M Yang Jing J Van Breusegem Frank F Messens Joris J

Proceedings of the National Academy of Sciences of the United States of America 20191002 42

Hydrogen peroxide (H2O2) is an important messenger molecule for diverse cellular processes. H2O2 oxidizes proteinaceous cysteinyl thiols to sulfenic acid, also known as S-sulfenylation, thereby affecting the protein conformation and functionality. Although many proteins have been identified as S-sulfenylation targets in plants, site-specific mapping and quantification remain largely unexplored. By means of a peptide-centric chemoproteomics approach, we ...[more]

PMID: 31578252

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Project description:Regulation of gene expression by reactive oxygen species (ROS) is an important component of abiotic stress signal transduction mechanisms. Previous genome-wide analysis of chilling-induced changes in gene expression suggested a potential role of H2O2 and other ROS as key signals in the induction of early response genes in japonica rice (cv. Nipponbare). Candidate regulators including bZIP-, Myb- or WRKY-types of transcription factors that were responsive to both chilling (10oC) and exogenous H2O2, and their probable target clusters (as1/ocs/TGA1-like, MybR-like element containing genes) were inferred by integrative analysis of qPCR expression profiles and ab initio promoter motif data. In an effort to define the composition and hierarchical organization of the ROS-mediated chilling response regulatory networks, we examined the effect of exogenous H2O2 (6 hours in 4mM H2O2 at 28oC and then 6 hours of recovery in water at 28oC) on the transcriptome of Nipponbare in a genome-wide scale and compared them with the chilling stress transcriptome. Results of this study were consistent with our earlier model that a ROSbZIP-as1/ocs/TGA1 regulatory module is a direct consequence of chilling-induced elevation of intracellular ROS during the initial 24 hours. Keywords: time course (response to exogenous H2O2) This experiment describes the analysis of the temporal changes in gene expression in response to exogenous H2O2 in Nipponbare rice. A total of 5 treatment regimes were examined (1, 3, and 6 hours at 4 mM H2O2 and then 3 and 6 hours of recovery in water after 6 hours of exposure to H2O2). The microarray platform used in this study was the 45K oligonucleotide microarray (www.ricearray.org) which comes in pairs of slides A and B (each containing about 50% of the total number of features). The data described in this series include all hybridizations with slide A and with slide B. All experiments were performed with two independent biological replicates (R1 and R2) for each time point.

			Action	DRS
	EOS438_chymo_01.mgf	Mgf
	EOS438_chymo_01.raw	Raw
	EOS438_chymo_01.xlsx	Xlsx
	EOS438_chymo_01_PSM.xlsx	Xlsx
	EOS438_chymo_02.mgf	Mgf

Dataset Information

Protein S-sulfenylation Arabidopsis - AtMAPK4

Dataset's files

Publications

Mining for protein S-sulfenylation in <i>Arabidopsis</i> uncovers redox-sensitive sites.

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