Dataset Information

Mass spectrometry of excretory/secretory products (ectosomes, exosomes, and soluble proteins) secreted by Toxoplasma gondii tachyzoites isolated from an infected murine model.

ABSTRACT: Toxoplasma gondii is an intracellular parasite that infects all nucleated cells; to date, this organism is considered as one of the most successful parasites in the world. During infection, internalization, and proliferation the parasite excretes/secretes a high number of proteins, several of them with identified roles, including MIC proteins, ROP proteins and GRA proteins involved in adhesion, invasion and intracellular proliferation respectively. The characterization of the secretome of Toxoplasma gondii opens the possibility to find new molecules for the design of future anti-parasitic strategies. The complete secretome and proteome of the parasite isolated from the murine model as well as their quantification by MS analysis are now available in the present report.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Toxoplasma Gondii Gt1

TISSUE(S): Excretion, Secretion

DISEASE(S): Toxoplasmosis

SUBMITTER: Emmanuel Rios-Castro

LAB HEAD: Ricardo Mondragón-Flores

PROVIDER: PXD013767 | Pride | 2019-08-29

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	20180809_PES_Tgondii_1.raw.zip	Raw
	20180809_PES_Tgondii_1_IA_final_peptide.csv	Csv
	20180809_PES_Tgondii_1_IA_final_protein.csv	Csv
	20180809_PES_Tgondii_2.raw.zip	Raw
	20180809_PES_Tgondii_2_IA_final_peptide.csv	Csv

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Project description:Tymoshenko2015 - Genome scale metabolic model - ToxoNet1 This model is described in the article: Metabolic Needs and Capabilities of Toxoplasma gondii through Combined Computational and Experimental Analysis. Tymoshenko S, Oppenheim RD, Agren R, Nielsen J, Soldati-Favre D, Hatzimanikatis V. PLoS Comput. Biol. 2015 May; 11(5): e1004261 Abstract: Toxoplasma gondii is a human pathogen prevalent worldwide that poses a challenging and unmet need for novel treatment of toxoplasmosis. Using a semi-automated reconstruction algorithm, we reconstructed a genome-scale metabolic model, ToxoNet1. The reconstruction process and flux-balance analysis of the model offer a systematic overview of the metabolic capabilities of this parasite. Using ToxoNet1 we have identified significant gaps in the current knowledge of Toxoplasma metabolic pathways and have clarified its minimal nutritional requirements for replication. By probing the model via metabolic tasks, we have further defined sets of alternative precursors necessary for parasite growth. Within a human host cell environment, ToxoNet1 predicts a minimal set of 53 enzyme-coding genes and 76 reactions to be essential for parasite replication. Double-gene-essentiality analysis identified 20 pairs of genes for which simultaneous deletion is deleterious. To validate several predictions of ToxoNet1 we have performed experimental analyses of cytosolic acetyl-CoA biosynthesis. ATP-citrate lyase and acetyl-CoA synthase were localised and their corresponding genes disrupted, establishing that each of these enzymes is dispensable for the growth of T. gondii, however together they make a synthetic lethal pair. This model is hosted on BioModels Database and identified by: MODEL1504280000. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Project description:Toxoplasma gondii is a globally distributed obligate intracellular parasite which can cause zoonotic toxoplasmosis with great harms. The average death time of mice that infected with Toxoplasma gondii RH strain tachyzoites recovered from the liquid nitrogen was shortened after multiple generations. It has been reported that the parasite is in a state of static virulence during cryopreservation and the virulence of the protozoan parasite can be enhanced after continuous passages in hosts under laboratory conditions. However, no research has been conducted to elucidate its biological mechanism. Herein, we sequenced the T. gondii transcriptome using RNA-Seq technology and performed de novo assembly to investigated the virulence factors expression changes by comparing gene expression profiles between incipiently recovered and completely resuscitated tachyzoites. Transcriptome analysis identified 1,951 differentially expressed transcripts in infected liver, of which 1,752 were significantly downregulated and 199 upregulated. We identified many differentially expressed proteins and genes, including serine/threonine kinase, calnexin, myosin and microtubule-associated protein which have previously been reported to be either involved in cell adhesion, parasite gliding or participate in cell invasion. The great majority of the virulence factors including microneme proteins, rhoptry proteins and dense granule proteins were upregulated in fully recovered tachyzoites. The enhanced virulence of recovered Toxoplasma gondii RH strain from the liquid nitrogen is associated with the up-regulated expression of MICs, ROPs and GRAs. Our data will facilitate future genomic research and in-depth annotation of Toxoplasma gondii RH strain genomes. This study provides a profile of the candidate genes that are suspected to be involved with virulence enhancement of recovered Toxoplasma gondii RH strain tachyzoites. Many further studies should be carried out to confirm the function of the candidate genes. Moreover, the preliminary identification of genes and pathways exhibiting differential expression in complete resuscitation stage may further our general understanding of virulence enhancement in this parasite.

Dataset Information

Mass spectrometry of excretory/secretory products (ectosomes, exosomes, and soluble proteins) secreted by Toxoplasma gondii tachyzoites isolated from an infected murine model.

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