Proteomics

Dataset Information

0

Meiosis-associated argonaute (MAGO) proteins are necessary for meiosis in maize

ABSTRACT: Plants do not specify their germline until late in their life cycle. Hence, the plant germline is normally specified from terminally differentiated somatic cells, though the precise mechanism(s) are unknown. We have found that male gametogenesis in maize is associated with the accumulation of distinct 21-nt phased small-interfering RNAs (phasiRNAs) generated by meiosis-associated argonaute (MAGO) proteins. MAGO1 accumulates in the epidermis of pre-meiotic anthers while MAGO2 is found in developing meiocytes. We have found that MAGO proteins are required for meiocyte development as mutants display chromosomal defects and male infertility. Furthermore, we detect the heat stress-induced activation of a distinct class of Long terminal repeat retrotransposons in the male germline of MAGO mutants. Our data suggests that MAGO proteins and the reproductive phasiRNAs play important roles protecting the germline from transposable elements during environmental stress conditions.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Zea Mays (maize)

TISSUE(S): Floret

SUBMITTER: Andrew Bottrill  

LAB HEAD: Dr Jose Gutierrez-Marcos

PROVIDER: PXD013891 | Pride | 2022-01-04

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
Experiment Scaffold search_C1_1 from OT_190410_23_MS19-091_C1_1.mzid_Experiment_Scaffold_search_C1_1_from_OT_190410_23_MS19-091_C1_1.pride.mgf.gz Mzid
Experiment Scaffold search_C1_1 from OT_190410_23_MS19-091_C1_1.pride.mztab.gz Mztab
Experiment Scaffold search_C1_2 from OT_190410_23_MS19-091_C1_2.mzid_Experiment_Scaffold_search_C1_2_from_OT_190410_23_MS19-091_C1_2.pride.mgf.gz Mzid
Experiment Scaffold search_C1_2 from OT_190410_23_MS19-091_C1_2.pride.mztab.gz Mztab
Experiment Scaffold search_C2_1 from OT_190410_23_MS19-091_C2_1.mzid_Experiment_Scaffold_search_C2_1_from_OT_190410_23_MS19-091_C2_1.pride.mgf.gz Mzid
Items per page:
1 - 5 of 120

Similar Datasets

Proteomics Endodermis-specific Receptor-like Kinase (ERK1) is required for the deposition of lignin in the Casparian strip in the root
2021-09-08 | PXD013861 | Pride
Proteomics Phosphoproteomic analysis of cell-based resistance to BRAF inhibitor therapy in melanoma
2018-10-24 | PXD002079 | Pride
Proteomics Fission yeast NDR/LATS kinase Orb6 regulates exocytosis via phosphorylation of exocyst complex
2019-01-31 | PXD009408 | Pride
Proteomics Phosphoproteome profiling uncovers a key role for CDKs in TNF signaling
2021-10-11 | PXD021366 | Pride
Proteomics A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo development
2024-08-28 | PXD054119 | Pride
Proteomics Overexpression of Lin28A in neural progenitor cells in vivo does not lead to brain tumor formation but results in reduced spine density
2022-02-15 | PXD028044 | Pride
Proteomics Phosphoproteomic analysis of the Folliculin (FLCN) tumor suppressor reveals FLCN-dependent kinase signaling and TFEB regulation
2022-07-11 | PXD025798 | Pride
Proteomics Quantitative proteomics analysis of Angiostrongylus vasorum-induced alterations in dog serum
2021-01-12 | PXD016881 | Pride
Proteomics Central role of phosphorylation in the circadian control of liver metabolism
2016-10-18 | PXD003751 | Pride
Proteomics MDP phosphorylation by NAGK is essential for NOD2 activation
2022-06-13 | PXD022384 | Pride