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The mitochondrial and chloroplast proteome of Euglena gracilis

ABSTRACT: Euglena gracilis is regarded as a flexible and adaptable free-living protist of considerable biotechnological interest. We carried out proteomic analysis of the E. gracilis purified chloroplast and mitochondrion. These data provide a new level of insight into functions and unique characteristics of these organelles and reveals the manner, in which these differ from its counterparts in other organisms.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Euglena Gracilis

TISSUE(S): Photosynthetic Cell

DISEASE(S): Disease Free

SUBMITTER: Martin Zoltner

LAB HEAD: Martin Zoltner

PROVIDER: PXD014767 | Pride | 2019-10-28

REPOSITORIES: Pride

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	CP1.raw	Raw
	CP2.raw	Raw
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	CP6.raw	Raw

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Publications

A Uniquely Complex Mitochondrial Proteome from Euglena gracilis.

Hammond Michael J MJ Nenarokova Anna A Butenko Anzhelika A Zoltner Martin M Dobáková Eva Lacová EL Field Mark C MC Lukeš Julius J

Molecular biology and evolution 20200801 8

Euglena gracilis is a metabolically flexible, photosynthetic, and adaptable free-living protist of considerable environmental importance and biotechnological value. By label-free liquid chromatography tandem mass spectrometry, a total of 1,786 proteins were identified from the E. gracilis purified mitochondria, representing one of the largest mitochondrial proteomes so far described. Despite this apparent complexity, protein machinery responsible for the extensive RNA editing, splicing, and proc ...[more]

PMID: 32159766

Publication: 1/2

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The distinctive flagellar proteome of Euglena gracilis

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Mitochondrial proteome from liver and intestinal organoids from WT, Nrf2 knockout and Keap1 knockdown mice

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DNA-seq of mitochondrial DNA from liver of OGG1 and MUTYH deficient mice that have been bred for five consecutive generations

Project description:Studying whether removal of base-excision repair from mitochondria will result into increase in mitochondrial DNA (mtDNA) mutation load. The endogenous genes of OGG1 and MUTYH DNA glycosylases were modified to lack the genomic region encoding for the predicted mitochondrial targeting sequence. The mouse lines used: A mouse line that lacks the region encoding for the mitochondrial targeting sequence (L2 to W23) of OGG1 (Ogg1 dMTS mice). A mouse line that lacks the region encoding the mitochondrial targeting sequence (K2 to P33) of MUTYH (Mutyh dMTS mice). To accumulate mutations to the mitochondrial DNA these mice were bred double homozygous Mutyh dMTS x Ogg1 dMTS mice as a maternal lineage for five consecutive generations and mitochondrial DNA from liver was extracted from the offspring and sequenced with Illumina. OGG1 and MUTYH are involved in repair of 8-oxo-dG from DNA. 8-oxo-dG can be a mutagenic lesion because some DNA repair polymerases are known to erroneously incorporate adenosine opposite to 8-oxo-dG during replication leading to GC>TA transversion mutations.

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