Dataset Information

Mass spectrometry analysis following chromatin fractionation in growing and senescent cells

ABSTRACT: This project contains the raw and result files for a mass spectrometry analysis comparing the abundance of proteins following chromatin fractionation in growing cells and in senescent cells for the WI-38hTERT/GFP-RAF1-ER cell line. Two replicas were analysed for each cell condition. The data are used to support a study on using polymer modelling to investigate the roles of heterochromatin and lamina interactions on chromosome reorganisation in senescence.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Diploid Cell, Fibroblast

SUBMITTER: Michael Chiang

LAB HEAD: Hisham Mohammed

PROVIDER: PXD014929 | Pride | 2019-09-25

REPOSITORIES: Pride

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Publications

Polymer Modeling Predicts Chromosome Reorganization in Senescence.

Chiang Michael M Michieletto Davide D Brackley Chris A CA Rattanavirotkul Nattaphong N Mohammed Hisham H Marenduzzo Davide D Chandra Tamir T

Cell reports 20190901 12

Lamina-associated domains (LADs) cover a large part of the human genome and are thought to play a major role in shaping the nuclear architectural landscape. Here, we perform polymer simulations, microscopy, and mass spectrometry to dissect the roles played by heterochromatin- and lamina-mediated interactions in nuclear organization. Our model explains the conventional organization of heterochromatin and euchromatin in growing cells and the pathological organization found in oncogene-induced sene ...[more]

PMID: 31533042

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			Action	DRS
	Tamir_TMT_F10_23.mgf	Mgf
	Tamir_TMT_F10_23.pride.mgf.gz	Mgf
	Tamir_TMT_F10_23.raw	Raw
	Tamir_TMT_F11_24.mgf	Mgf
	Tamir_TMT_F11_24.pride.mgf.gz	Mgf