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Multiomics Analyses Reveal Dynamic Bioenergetic Pathways and Functional Remodeling of the Heart During Intermittent Fasting

ABSTRACT: Intermittent fasting (IF) reduces cardiovascular risk factors in animals and humans, and can protect the heart against ischemic injury in models of myocardial infarction, but the underlying molecular mechanisms are unknown. To delineate molecular perturbations in response to IF, we carried out comprehensive analyses of molecular pathways and biological processes using proteomic and phosphoproteomic data followed by functional analysis of hearts from mice maintained for 6 months on either daily 12- or 16-hour fasting, every-other-day fasting or ad libitum control feeding regimens. IF regimens significantly affected pathways that regulate cyclic GMP signaling, lipid and amino acid metabolism, cell adhesion, cell death and inflammation. Comparison of differentially expressed proteome and transcriptome upon IF showed higher correlation of pathway alternation in early IF regimen, but inverse correlation of metabolic processes such as fatty acid oxidation and immune processes in later IF regimens. Echocardiographic analyses demonstrated that IF enhances stress-induced cardiac performance. In addition to providing a valuable resource, our systemic analyses reveal molecular framework for understanding how IF impacts the function of the heart and its vulnerability to injury and disease.

INSTRUMENT(S): Q Exactive Plus, Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Asfa Alli Shaik

LAB HEAD: Jayantha GUNARATNE

PROVIDER: PXD015452 | Pride | 2023-09-27

REPOSITORIES: pride

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			Action	DRS
	TBP_QEP_P5_180411_01_IS1126_Set2_bRP1.raw	Raw
	TBP_QEP_P5_180411_02_IS1126_Set2_bRP2.raw	Raw
	TBP_QEP_P5_180411_03_IS1126_Set2_bRP3.raw	Raw
	TBP_QEP_P5_180411_04_IS1126_Set2_bRP4.raw	Raw
	TBP_QEP_P5_180411_05_IS1126_Set2_bRP5.raw	Raw

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Multiomics Analyses Reveal Dynamic Bioenergetic Pathways and Functional Remodeling of the Heart During Intermittent Fasting

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