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Human Heart Whole Tissue Lysate LC-MS/MS

ABSTRACT: Commercial human heart whole tissue lysates were analyzed with LC-MS/MS with an inclusion list of alternative sequences (Lau et al. bioRxiv 2019).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart

SUBMITTER: Maggie Pui Yu Lam

LAB HEAD: Maggie Pui Yu Lam

PROVIDER: PXD015544 | Pride | 2019-12-16

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20190827_HumanLysate3ug_HeavyPep1pm_F10.mzML	Mzml
	20190827_HumanLysate3ug_HeavyPep1pm_F1_190827145748.mzML	Mzml
	20190827_HumanLysate3ug_HeavyPep1pm_F2.mzML	Mzml
	20190827_HumanLysate3ug_HeavyPep1pm_F3.mzML	Mzml
	20190827_HumanLysate3ug_HeavyPep1pm_F4.mzML	Mzml

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Publications

Splice-Junction-Based Mapping of Alternative Isoforms in the Human Proteome.

Lau Edward E Han Yu Y Williams Damon R DR Thomas Cody T CT Shrestha Rajani R Wu Joseph C JC Lam Maggie P Y MPY

Cell reports 20191201 11

The protein-level translational status and function of many alternative splicing events remain poorly understood. We use an RNA sequencing (RNA-seq)-guided proteomics method to identify protein alternative splicing isoforms in the human proteome by constructing tissue-specific protein databases that prioritize transcript splice junction pairs with high translational potential. Using the custom databases to reanalyze ∼80 million mass spectra in public proteomics datasets, we identify more than 1, ...[more]

PMID: 31825849

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