Proteomics

Dataset Information

0

The structure and symmetry of the Radial Spoke protein complex in Chlamydomonas flagella


ABSTRACT: Chemical cross-linking coupled to mass spectrometry was used to study the organization of the Radial Spoke protein complex from Chlamydomonas. Experiments were performed by on-bead cross-linking of the immobilized complex with the amine-reactive cross-linking reagent disuccinimidyl suberate (DSS).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Chlamydomonas Reinhardtii

SUBMITTER: Alexander Leitner  

LAB HEAD: Takashi Ishikawa

PROVIDER: PXD015658 | Pride | 2020-08-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
RSP_identifications.xlsx Xlsx
aleitner_D1612_036.raw Raw
aleitner_D1612_037.raw Raw
aleitner_D1612_038.raw Raw
aleitner_D1612_039.raw Raw
Items per page:
1 - 5 of 9
altmetric image

Publications

The structure and symmetry of the radial spoke protein complex in <i>Chlamydomonas</i> flagella.

Poghosyan Emiliya E   Iacovache Ioan I   Faltova Lenka L   Leitner Alexander A   Yang Pinfen P   Diener Dennis R DR   Aebersold Ruedi R   Zuber Benoit B   Ishikawa Takashi T  

Journal of cell science 20200821 16


The radial spoke is a key element in a transducer apparatus controlling the motility of eukaryotic cilia. The transduction biomechanics is a long-standing question in cilia biology. The radial spoke has three regions - a spoke head, a bifurcated neck and a stalk. Although the neck and the stalk are asymmetric, twofold symmetry of the head has remained controversial. In this work we used single particle cryo-electron microscopy (cryo-EM) analysis to generate a 3D structure of the whole radial spo  ...[more]

Similar Datasets

2019-07-15 | PXD013470 | Pride
2018-09-10 | PXD009106 | Pride
2020-07-07 | PXD013907 | Pride
2021-06-28 | PXD024131 | Pride
2018-06-11 | PXD009526 | Pride
2018-07-26 | PXD007185 | Pride
2021-02-04 | PXD019751 | Pride
2020-06-18 | PXD015682 | Pride
2022-10-21 | PXD020636 | Pride
2020-01-16 | PXD015394 | Pride