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Mass spectrometry of Manica rubida venom

ABSTRACT: In the present study, the venom peptidome of the red ant, Manica rubida, was characterized using an integrated transcriptomic and proteomic approach. In addition, insecticidal assays on C18 reversed-phase HPLC venom fractions were conducted on Lucilia caesar blowflies to identify neurotoxic peptides.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Manica Rubida

TISSUE(S): Venom Sac

SUBMITTER: Axel Touchard

LAB HEAD: Michel Treilhou

PROVIDER: PXD015675 | Pride | 2020-03-20

REPOSITORIES: Pride

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Publications

Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida: Identification of Insecticidal Toxins.

Touchard Axel A Aili Samira R SR Téné Nathan N Barassé Valentine V Klopp Christophe C Dejean Alain A Kini R Manjunatha RM Mrinalini Coquet Laurent L Jouenne Thierry T Lefranc Benjamin B Leprince Jérôme J Escoubas Pierre P Nicholson Graham M GM Treilhou Michel M Bonnafé Elsa E

Journal of proteome research 20200324 4

Using an integrated transcriptomic and proteomic approach, we characterized the venom peptidome of the European red ant, Manica rubida. We identified 13 "myrmicitoxins" that share sequence similarities with previously identified ant venom peptides, one of them being identified as an EGF-like toxin likely resulting from a threonine residue modified by O-fucosylation. Furthermore, we conducted insecticidal assays of reversed-phase HPLC venom fractions on the blowfly Lucilia caesar ...[more]

PMID: 32182430

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