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Starvation-specific NSPs in Leishmania mexicana parasites by quantitative proteomics

ABSTRACT: Using a combination of BONCAT technique and iTRAQ 3-plex quantitative proteomics MS, we profiled the de novo protein synthesis in the starving L. mexicana parasites in a temporally resolved manner. This approach uniquely integrates the high specificity of the BONCAT technique for the NSPs with the high sensitivity and multiplexed quantitation capability of the iTRAQ quantitative proteomics MS. Proof-of-concept experiments identified over 250 starvation-specific NSPs in the parasite and quantified the relative changes in abundance of these proteins as a function of duration of starvation.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Trypanosoma Brucei

TISSUE(S): Primary Cell

SUBMITTER: Kalesh Karunakaran

LAB HEAD: Dr Kalesh Karunakaran

PROVIDER: PXD015827 | Pride | 2019-12-04

REPOSITORIES: Pride

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			Action	DRS
	3-10-19_Kalesh_iTRAQ_A.wiff	Wiff
	3-10-19_Kalesh_iTRAQ_B.wiff	Wiff
	proteinGroups.txt	Txt

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Publications

A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation.

Kalesh Karunakaran K Denny Paul W PW

PLoS neglected tropical diseases 20191219 12

Adaptation to starvation is integral to the Leishmania life cycle. The parasite can survive prolonged periods of nutrient deprivation both in vitro and in vivo. The identification of parasite proteins synthesised during starvation is key to unravelling the underlying molecular mechanisms facilitating adaptation to these conditions. Additionally, as stress adaptation mechanisms in Leishmania are linked to virulence as well as infectivity, profiling of the complete repertoire of Newly Synthesised ...[more]

PMID: 31856154

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