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Comparative gene expression profiling of mutants of Amycolatopsis mediterranei S699

ABSTRACT: The project aims to unveil the mechanism of rifamycin and its analog production by wild type A. mediterranei S699 and mutant A. mediterranei DCO#34 known to produce 24-desmethylrifamycin B at two time points. Stain that donot produce this molecule is taken as the negative control in the study.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Amycolatopsis Mediterranei S699

SUBMITTER: RUP LAL

LAB HEAD: Rup Lal

PROVIDER: PXD016416 | Pride | 2021-03-11

REPOSITORIES: Pride

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			Action	DRS
	20171031_OP_DU_AM_13_1.raw	Raw
	20171031_OP_DU_AM_13_2.raw	Raw
	20171031_OP_DU_AM_13_3.raw	Raw
	20171031_OP_DU_AM_13_4.raw	Raw
	20171031_OP_DU_AM_17_1.raw	Raw

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Publications

Differential mass spectrometry-based proteome analyses unveil major regulatory hubs in rifamycin B production in Amycolatopsis mediterranei.

Singhvi Nirjara N Singh Priya P Prakash Om O Gupta Vipin V Lal Sukanya S Bechthold Andreas A Singh Yogendra Y Singh Rakesh Kumar RK Lal Rup R

Journal of proteomics 20210302

Rifamycin B is produced by Amycolatopsis mediterranei S699 as a secondary metabolite. Its semi-synthetic derivatives have been used for curing tuberculosis caused by Mycobacterium tuberculosis. But the emergence of rifampicin-resistant strains required analogs of rifamycin B to be developed by rifamycin biosynthetic gene cluster manipulation. In 2014 genetic engineering of the rifamycin polyketide synthase gene cluster in S699 led to a mutant, A. mediterranei DCO#34, that produced 24-desmethylri ...[more]

PMID: 33662614

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