Identifying mTOR regulated RNA binding proteins
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ABSTRACT: To investigate the RNA binding proteins that change following mTOR inhibition, HeLa cells were treated with and without 200nM Torin1 for an hour. Cells were the UV254 cross-linked, lysed and large scale whole cell oligo(dT) based pulldowns were performed with identification and quantification of purified proteins by label free mass spectrometry.
INSTRUMENT(S): Synapt MS
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell, Hela Cell
DISEASE(S): Cervix Carcinoma
SUBMITTER: Rebekah Jukes-Jones
LAB HEAD: Martin Bushell
PROVIDER: PXD016705 | Pride | 2020-11-24
REPOSITORIES: Pride
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