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Proteomic analysis of Acinetobacter baylyi ADP1 under exposure of disinfectants

ABSTRACT: The goals of this study are to use SWATH-MS to detect bacterial proteomic profiles of wild-type Acinetobacter baylyi ADP1, and its protein response under the exposure of disinfectants, including chloramine and free chlorine. The concentrations of disinfectants were 10 mg/L. The group without dosing disinfectant was the control group. Each concentration was conducted in triplicate. By comparing the proteomic profiles of experimental groups and control group, the effects of disinfectants on translational levels can be revealed.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Acinetobacter Sp.

SUBMITTER: Jianhua Guo

LAB HEAD: Jianhua Guo

PROVIDER: PXD016794 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20180928_AWMC_Yue_IDA_NH2Cl.wiff	Wiff
	20180928_AWMC_Yue_IDA_NH2Cl.wiff.scan	Wiff
	20180928_AWMC_Yue_IDA_NH2ClCOD.wiff	Wiff
	20180928_AWMC_Yue_IDA_NH2ClCOD.wiff.scan	Wiff
	20180928_AWMC_Yue_IDA_NaClO.wiff	Wiff

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1 - 5 of 49

Publications

Chlorine disinfection facilitates natural transformation through ROS-mediated oxidative stress.

Zhang Shuai S Wang Yue Y Lu Ji J Yu Zhigang Z Song Hailiang H Bond Philip L PL Guo Jianhua J

The ISME journal 20210503 10

The bacterial infection that involves antimicrobial resistance is a rising global threat to public health. Chlorine-based water disinfection processes can inactivate antibiotic resistant bacteria. However, at the same time, these processes may cause the release of antibiotic resistance genes into the water as free DNA, and consequently increase the risk to disseminate antibiotic resistance via natural transformation. Presently, little is known about the contribution of residual chlorine affectin ...[more]

PMID: 33941886

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Proteomic analysis of Acinetobacter baylyi ADP1 under exposure of non-antibiotic pharmaceuticals

Project description:The goals of this study are to use SWATH-MS to detect bacterial proteomic profiles of wild-type Acinetobacter baylyi ADP1, and its protein response under the exposure of non-antibiotic pharmaceuticals, including ibuprofen, naproxen, gemfibrozil, diclofenac, propanolol, and iopromide. The concentrations were 0.5 mg/L for ibuprofen, naproxen, gemfibrozil, diclofenac, propanolol, and 1.0 mg/L for iopromide. The group without dosing pharmaceutical was the control group. Each concentration was conducted in triplicate. By comparing the proteomic profiles of experimental groups and control group, the effects of non-antibiotic pharmaceuticals on translational levels can be revealed.

2020-05-22 | PXD016798 | Pride

Proteomic analysis of E. coli K-12 LE392, P. putida KT2440 under exposure of carbamazepine

Project description:The goals of this study are to use SWATH-MS to detect bacterial proteomic profiles of wild-type E. coli K-12 LE392, P. putida KT2440, and their quantitative protein response under the exposure of antiepileptic drug carbamazepine. Three concentrations of carbamazepine were applied, which were 0.05 mg/L, 10.0 mg/L and 50.0 mg/L. The group without dosing carbamazepine was the control group. Each concentration was conducted in triplicate. By comparing the proteomic profiles of experimental groups and control group, the effects of carbamazepine on bacterial translational levels can be revealed.

2018-10-10 | PXD009279 | Pride

Proteomic analysis of E. coli K-12 LE392, P. putida KT2440 under exposure of six non-antibiotic pharmaceuticals

Project description:The goals of this study are to use SWATH-MS to detect bacterial proteomic profiles of E. coli K-12 LE392 with conjugative RP4 plasmid, P. putida KT2440, and their protein response under the exposure of six kinds of non-antibiotic pharmaceuticals, i.e., ibuprofen (ibu), naproxen (nap), gemfibrozil (gem), iopromide (iop), diclofenac (dic), propanolol (pro). Each treatment condition was conducted in triplicate. By comparing the proteomic profiles of experimental groups and control group, the effects of these non-antibiotic pharmaceuticals on translational levels can be revealed.

2022-02-15 | PXD012642 | Pride

Mechanism of persistence of the ammonia oxidising bacteria - Nitrosomonas to the the biocide Free Nitrous Acid (FNA)

Project description:This study shows the molecular mechanism by which the ammonia oxidising bacteria- Nitrosomonas is able to persist in high concentrations of FNA.

2023-06-02 | PXD007514 | Pride

Use of SWATH mass spectrometry for quantitative proteomic investigation of Shewanella oneidensis MR-1 biofilms grown on graphite cloth electrodes

Project description:. In this study we show successful use of SWATH-MS for quantitative proteomic analysis of a microbial electrochemically active biofilm. Shewanella oneidensis MR-1 was grown on carbon cloth electrodes under continuous anodic electrochemical polarizations in a bioelectrochemical system. Using lactate as the electron donor, anodes serving as terminal microbial electron acceptors were operated at three different electrode potentials (+0.71V, +0.21V & -0.19V vs. SHE) and the development of catalytic activity was monitored by measuring the current traces over time. Once maximum current was reached (usually within 21-29 hours) the electrochemical systems were shut off and biofilm proteins were extracted from the electrodes for proteomic assessment.

2016-08-10 | PXD001472 | Pride

Transcriptomic Profiling of Salmonella enterica Enteritidis and Typhimurium under Chlorine Based Oxidation

Project description:Investigation of whole genome gene expression level changes in Salmonella enterica serova Enteritidis and Typhimurium under chlorine treatment An eighteen chip study using total RNA isolated from three separate cultures of (1) S. Enteritidis in BHI broth (2) S. Typhimurium in BHI broth (3) S. Enteritidis in BHI broth w/ 130 ppm chlorine (4) S. Typhimurium in BHI w/ 130 ppm chlorine (5) S. Enteritidis in BHI broth w/ 390 ppm (6) S. Typhimurium in BHI broth w/ 390 ppm. Each chip measures the expression level of 5,027 ORFs covering the whole genome of S. Enteritidis and S. Typhimurium.

2010-05-06 | E-GEOD-20701 | biostudies-arrayexpress

Gene expression in developing fibrotic lesions in tracheas of chlorine-exposed mice

Project description:Chlorine is a widely used industrial chemical that is also considered a chemical threat agent. Inhalation of chlorine gas can cause acute injury to the respiratory tract, including the death of airway epithelial cells. Failure to efficiently repair the epithelial damage is associated with long-term respiratory abnormalities, including airway fibrosis. We previously developed a model of airway injury in which mice exposed to chlorine gas exhibit epithelial damage and develop fibrosis in large airways. In the present study, we measured gene expression in developing fibrotic lesions isolated from chlorine-exposed mice 4 days after exposure and compared to expression in corresponding areas from unexposed mice. Mesenchymal tissue was isolated by laser-capture microdissection to limit the analysis to the developing fibrotic lesions. The 4-day time point was chosen in an attempt to identify early profibrotic signaling events because at this time fibroblast proliferation has commenced but the fibrotic scar has not yet formed.

2018-01-19 | GSE109365 | GEO

Transcriptome Analysis of T3SS1 in Vibrio parahaemolyticus

Project description:Comparative transcriptional mRNA profiles were generated of bacterial pathogen Vibrio parahaemolyticus under conditions that induce activity of virulence factor type III secretion system 1 (T3SS1). Induction conditions included growth of the bacteria in Dulbecco's Modified Eagle Medium (DMEM), overexpression of transcriptional activator exsA and contact with HeLa cells in Hank's Balanced Salt Solution (HBSS), while non-inducing conditions included growth in Luria-Bertani medium supplemented with 2.5% (w/v) NaCl (LB-S) and overexpression of transcriptional anti-activator exsD. Transcriptome profiles of induction conditions were cross-compared against background non-inducing conditions and time points during HeLa cell infection (2 hr, 3 hr, 4 hr, 6 hr, 8 hr) were compared against pre-infection (0 hr) to identify genes important in T3SS1 activity and pathogenesis. Duplicate mRNA transcriptome profiles of V. parahaemolyticus grown in T3SS1 inducing (DMEM, exsA expression) and non-inducing (LB-S, exsD expression) conditions as well as during HeLa cell infection (0 hr, 2 hr, 3 hr, 4 hr, 6 hr, 8 hr) were generated by deep sequencing using an Illumina MiSeq

2014-01-21 | E-GEOD-51423 | biostudies-arrayexpress

Retinal proteomic analysis in a mouse model of endotoxin-induced uveitis using a data-independent acquisition-based mass spectrometry

Project description:Endotoxin-induced uveitis (EIU) in rodents is a good animal model to study uveitis and associated acute retinal inflammation. To understand the pathogenic mechanism of uveitis and screen the potential targets for treatment, we analyzed the retinal proteomic profiles of EIU and normal C57BL/6J mice using a data-independent acquisition-based mass spectrometry (SWATH-MS).

2022-08-12 | PXD033532 | Pride

Transcription profiling of E. coli O157

Project description:Minimal processing using chlorinated water washes is a common practice in the fresh produce industry to reduce the microbial load and bacterial pathogens attached on produce surfaces. To evaluate if E. coli O157:H7 strains with different phyllogenetic backgrounds are equally sensitive or display variable resistance to chlorine treatment, we studied the expression profile of Sakai and one 2006 spinach outbreak strain (TW 14359) in response to chlorine and hydrogen peroxide treatment. Experiment Overall Design: 2 E. coli O157 strains are incubated with or without chlorine or H2O2 for 30 min for RNA extraction and hybridization on Affymetrix microarrays. We sought to compare the expression profile between the 2 strains with or without oxidative stress.

2009-04-11 | E-GEOD-15533 | biostudies-arrayexpress

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