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Identification of RNA binding Proteins in A. thaliana leaves

ABSTRACT: The goal of this MS project was to identify the RNA binding proteins in Arabidopsis thaliana leaves. To this end RBPs were isolated by a technique termed ‘RNA interactome capture’ and the captured proteins identified by mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Farnusch Kaschani

LAB HEAD: Farnusch Kaschani

PROVIDER: PXD018141 | Pride | 2020-05-26

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	ACE_0377_ARA_GCF_000001735.4_TAIR10.1_protein.fasta	Fasta
	ACE_0377_MBP09.raw	Raw
	ACE_0377_MBP10.raw	Raw
	ACE_0377_MBP11.raw	Raw
	ACE_0377_MBP12.raw	Raw

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Publications

Discovering the RNA-Binding Proteome of Plant Leaves with an Improved RNA Interactome Capture Method.

Bach-Pages Marcel M Homma Felix F Kourelis Jiorgos J Kaschani Farnusch F Mohammed Shabaz S Kaiser Markus M van der Hoorn Renier A L RAL Castello Alfredo A Preston Gail M GM

Biomolecules 20200424 4

RNA-binding proteins (RBPs) play a crucial role in regulating RNA function and fate. However, the full complement of RBPs has only recently begun to be uncovered through proteome-wide approaches such as RNA interactome capture (RIC). RIC has been applied to various cell lines and organisms, including plants, greatly expanding the repertoire of RBPs. However, several technical challenges have limited the efficacy of RIC when applied to plant tissues. Here, we report an improved version of RIC tha ...[more]

PMID: 32344669

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