Proteomics

Dataset Information

0

Re-annotated role of beta-Galactosidase in Flagellin deglycosylation

ABSTRACT: Most Eukaryotes recognise flagellin as a signature of bacterial invasion. In contrast to animals, plants do not recognise flagellin proteins, but conserved peptides released from flagellin (Felix et al., 1999). However, these peptides (e.g. flg22) are folded and buried deeply inside the flagellin polymer and would need to be released before they can interact with cell surface receptors, such as FLS2 (Fliegman & Felix, 2016). Here we discovered that the hydrolytic pathway releasing the flagellin elicitor in plants is initiated by a host-secreted beta-galactosidase (BGAL), which removes the terminal modified viosamine (mVio) from the O-glycan that cloaks the flagellin polymer. BGAL contributes to flagellin-dependent immunity but only against bacterial Pseudomonas syringae strains that carry mVio. Signatures of arms races at this new level of antagonistic interactions are that BGAL is suppressed during infection by a heat stable metabolite secreted by bacteria, and that other P. syringae strains carry BGAL-insensitive O-glycans.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Pseudomonas Syringae Pv. Tomato Nicotiana Benthamiana

SUBMITTER: Farnusch Kaschani  

LAB HEAD: Farnusch Kaschani

PROVIDER: PXD010461 | Pride | 2019-04-15

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
ACE_0058_BC05.raw Raw
ACE_0058_BC06.raw Raw
ACE_0058_BC07.raw Raw
ACE_0058_BC08.raw Raw
ACE_0058_BC13.raw Raw
Items per page:
1 - 5 of 11
altmetric image

Publications

Plants and animals recognize conserved flagellin fragments as a signature of bacterial invasion. These immunogenic elicitor peptides are embedded in the flagellin polymer and require hydrolytic release before they can activate cell surface receptors. Although much of flagellin signaling is understood, little is known about the release of immunogenic fragments. We discovered that plant-secreted β-galactosidase 1 (BGAL1) of <i>Nicotiana benthamiana</i> promotes hydrolytic elicitor release and acts  ...[more]

Similar Datasets

Proteomics Identification of flagellin peptides in N. benthamiana apoplastic fluid
2019-04-15 | PXD011823 | Pride
Proteomics Multi-omics revealed molecular mechanisms underlying guard cell systemic acquired resistance
2021-09-09 | PXD022880 | Pride
Proteomics Harnessing the acceptor substrate promiscuity of Clostridium botulinum Maf glycosyltransferase to glyco-engineer mini-flagellin protein chimeras
2024-08-22 | PXD044191 | Pride
Proteomics Repertoire and abundance of secreted virulence factors shape the pathogenic capacity of Pseudomonas syringae pv. aptata
2023-06-02 | PXD040715 | Pride
Transcriptomics Transcriptional profile of Pseudomonas syringae pv phaseolicola at low temperature
2012-12-31 | E-GEOD-38423 | biostudies-arrayexpress
Proteomics Released peptide from flagellin cleavage by SBT5.2a
2024-11-02 | PXD056369 | Pride
Transcriptomics The tomato receptor-like cytoplasmic kinase Fir1 is involved in flagellin signaling and regulates pre-invasion immunity
2022-11-28 | GSE199518 | GEO
Proteomics Triggering the polyurethane and polyethylene-degrading potential of filamentous fungi secretomes
2023-04-27 | PXD038835 | Pride
Proteomics Identification of O-glycosylation sites in Clostridium botulinum and Geobacillus kaustophilus flagellins
2020-09-07 | PXD018167 | Pride
Genomics HBI1 integrates hormonal and environmental signals to regulate the trade-off between growth and immunity (ChIP-seq)
2014-05-30 | E-GEOD-53099 | biostudies-arrayexpress