Proteomics

Dataset Information

0

Formaldehyde derivatization, a Side Reaction During Filter-Aided Sample Preparation

ABSTRACT: The filter-aided sample preparation (FASP) method has been commonly used for proteomic sample preparation due to its high efficiency and convenience. Herein, we report an overlooked side reaction during FASP method, resulting in a mass increment of 12 Da. We confirmed that the side reaction is caused by formaldehyde released from the spin filter and devel-oped a pretreatment step to reduce the side reaction. We further examined spin filters from different vendors and offered suggestions for different experimental purpose. Our findings would benefit the deep screening of labeling proteomics analy-sis and offer a new direction to improve performance of filter devices used for different biological and proteomics studies.

INSTRUMENT(S): Orbitrap Fusion Lumos, LTQ Orbitrap Elite

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

SUBMITTER: Zhen Wu  

LAB HEAD: Xumin Zhang

PROVIDER: PXD018737 | Pride | 2020-08-20

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
Lumos01534_XM_TLL_BSA_TEAB_Wash3_Amicon_mixed_Recal.msf Msf
Lumos01534_XM_TLL_BSA_TEAB_Wash3_Amicon_mixed_Recal.raw Raw
Lumos01535_XM_TLL_BSA_TEAB_Wash3_Microcon_mixed_Recal.msf Msf
Lumos01535_XM_TLL_BSA_TEAB_Wash3_Microcon_mixed_Recal.raw Raw
Lumos01536_XM_TLL_BSA_TEAB_Wash3_Sart_mixed_Recal.msf Msf
Items per page:
1 - 5 of 60
altmetric image

Publications

Formaldehyde Derivatization: An Unexpected Side Reaction During Filter-Aided Sample Preparation.

Tang Langlang L   Wu Zhen Z   Wang Jie J   Zhang Xumin X  

Analytical chemistry 20200828 18

The filter-aided sample preparation (FASP) method has been commonly used for proteomic sample preparation due to its high efficiency in removing impurities. Herein, we report an overlooked +12 Da side modification during FASP method using Microcon spin filters. We confirmed that the side modification is caused by formaldehyde released from the spin filter and found that the side modification leads to 10.5% and 9.5% loss in proteome-level peptide and protein identification, respectively. We evalu  ...[more]

Similar Datasets

Proteomics Systematic optimization of C-TAILS approach for deep screening of C-terminomics
2015-09-15 | PXD002409 | Pride
Proteomics Absolute quantitation of proteins by two-point quantitation using dimethylated proteotypic peptides
2018-11-27 | PXD007894 | Pride
Proteomics AtLEGbeta / gamma protease specificity characterization by PICS
2020-07-29 | PXD019220 | Pride
Proteomics An Undesirable Side Effect of EDC-catalyzed Carboxyl Labeling Approach
2020-12-10 | PXD020042 | Pride
Proteomics Barley leaf sample preparation
2018-09-03 | PXD008938 | Pride
Proteomics ATOMS determination of MMP cleavage site locations in YRS
2019-05-29 | PXD013366 | Pride
Transcriptomics Analysis of RNA distribution between pseudopodia and cell bodies in response to LPA stimulation
2008-03-18 | GSE10226 | GEO
Proteomics S-Trap is an ultrafast sample preparation approach for shotgun proteomics
2019-03-28 | PXD010237 | Pride
Proteomics Sirtilin protease specificity - Sirtilins - the new old members of the vitamin K-dependent coagulation factor family
2019-02-22 | PXD010679 | Pride
Proteomics Egg White as a quality control in MALDI Mass Spectrometry Imaging
2019-12-11 | PXD014310 | Pride