Proteomics

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PUB11-dependent ubiquitination of ALA10

ABSTRACT: Biogenesis of photosynthetic membranes depends on galactolipid synthesis, which relies on several cell compartments, notably the ER (endoplasmic reticulum) and the chloroplast envelope. Galactolipid synthesis involves lipid trafficking between both membrane compartments. In Arabidopsis, ALA10, a phospholipid flippase of the P4 type-ATPase family, has a positive effect on leaf development, interestingly counteracting the limitation of monogalactosyldiacylglycerol (MGDG) production. ALA10 locates in distinct domains of the ER depending on the ALIS (ALA interacting subunit) subunit it interacts with: close to the plasma membrane with ALIS1, or next to chloroplasts with ALIS5. It interacts with FAD2 (Fatty acid desaturase 2) and prevents accumulation of linolenic (18:3) containing phosphatidylcholine (PC) stimulating an increase of MGDG synthesis. Here we report that ALA10 interacts with PUB11 (plant U-box type 11), an E3 protein ubiquitin ligase, is ubiquitinated and degraded by the 26S proteasome in a PUB11 independent process.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Juliette Jouhet  

LAB HEAD: Juliette Jouhet

PROVIDER: PXD019412 | Pride | 2020-08-10

REPOSITORIES: Pride

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R1.mgf Mgf
R1.mzid.gz Mzid
R1.pride.mgf.gz Mgf
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Publications

PUB11-Dependent Ubiquitination of the Phospholipid Flippase ALA10 Modifies ALA10 Localization and Affects the Pool of Linolenic Phosphatidylcholine.

Salvaing Juliette J   Botella César C   Albrieux Catherine C   Gros Valérie V   Block Maryse A MA   Jouhet Juliette J  

Frontiers in plant science 20200715

Biogenesis of photosynthetic membranes depends on galactolipid synthesis, which relies on several cell compartments, notably the endoplasmic reticulum (ER) and the chloroplast envelope. Galactolipid synthesis involves lipid trafficking between both membrane compartments. In <i>Arabidopsis</i>, ALA10, a phospholipid flippase of the P<sub>4</sub> type-ATPase family, counteracts the limitation of monogalactosyldiacylglycerol (MGDG) production and has a positive effect on leaf development. ALA10 loc  ...[more]

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