Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Matrisome proteins of human and porcine cornea and amnion

ABSTRACT: In this project, for the first time, the Extracellular Matrix protein compositions of porcine cornea and human amnion (amniotic membrane) were characterized using LC/MS-MS based on an already developed protocol, "Matrisome Project".

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human) Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Cornea, Amnion

SUBMITTER: George Chlipala

LAB HEAD: Ali Djalilian

PROVIDER: PXD020606 | Pride | 2021-05-21

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	295-1.mgf	Mgf
	295-1.mzid.gz	Mzid
	295-1.raw	Raw
	295-2.mgf	Mgf
	295-2.mzid.gz	Mzid

Items per page:

1 - 5 of 12

Publications

Fabrication, Rheological, and Compositional Characterization of Thermoresponsive Hydrogel from Cornea.

Yazdanpanah Ghasem G Jiang Yizhou Y Rabiee Behnam B Omidi Meisam M Rosenblatt Mark I MI Shokuhfar Tolou T Pan Yayue Y Naba Alexandra A Djalilian Ali R AR

Tissue engineering. Part C, Methods 20210501 5

Fabricating thermoresponsive hydrogels from decellularized tissues is a trending and promising approach to develop novel biomaterials for tissue engineering and therapeutic purposes. There are differences in the characteristics of the produced hydrogels related to the source tissue as well as the decellularization and solubilization protocols used. Detailed characterization of the hydrogels will support the efforts to optimize their application as biomaterials for tissue engineering and therapeu ...[more]

PMID: 33813860

Similar Datasets

Matrisome of neonatal mouse dermal fibroblasts

Project description:The goal of this project is to compare the protein composition of the extracellular matrices (ECMs) deposited in vitro by wild type, heterozygous, or SPAG17 KO neonatal mouse dermal fibroblasts.

2022-09-22 | PXD019869 | Pride

ECM of proliferative vs dormant HNSCC

Project description:The goal of this project is to characterize the ECM composition of actively proliferative and dormant head and neck squamous cell carcinoma xenografts and identify components of the ECM niche instructing tumor cell dormancy.

2021-08-26 | PXD019185 | Pride

ECM of proliferative vs dormant HNSCC

2021-08-26 | PXD018883 | Pride

ECM of proliferative vs dormant HNSCC

2022-09-06 | PXD036453 | Pride

Xrn1 biochemically associates to the eisosome after the post diauxic shift in yeast

Project description:mRNA degradation is one of the main steps of gene expression, and a key player is the 5’-3’ exonuclease Xrn1. In Saccharomyces cerevisiae, it was previously shown, by a microscopy approach, that Xrn1 is located to different cellular compartments, depending on physiological state. During exponential growth, Xrn1 is distributed in the cytoplasm, while it is present in the eisosomes after the post-diauxic shift (PDS). Here, we biochemically characterized the Xrn1-associated complexes in different cellular states. We demonstrate that, after PDS, Xrn1 but not the decapping (DCP), nor Lsm1-7/Pat1 complexes, was sequestered in the eisosomes, thus preserving mRNAs from degradation.

2023-09-15 | PXD044514 | Pride

Xrn1 biochemically associates to the eisosome after the post diauxic shift in yeast (3)

2023-09-15 | PXD044634 | Pride

Xrn1 biochemically associates to the eisosome after the post diauxic shift in yeast (2)

2023-09-15 | PXD044631 | Pride

Xrn1 biochemically associates to the eisosome after the post diauxic shift in yeast (4)

2023-09-15 | PXD044653 | Pride

Extracellular matrix derived from oral mesenchymal stem cells

Project description:To investigate the different signatures of ECM proteins on GSCs and DPSCs as well as the different signatures of ECM proteins during osteogenic differentiation

2024-05-24 | PXD040575 | Pride

Dental Pulp Stem Cells LC-MS/MS analysis

Project description:This study focuses on the impact of ECM (extra-cellular matrix) over cell processes such as proliferation, differentiation or mineralization which is more specifically related to our dental pulp cells. We cultured these dental pulp stem cells (DPSC) in mineralization growth or normal growth conditions. After 21 days, cells were incubated in decellularized solution until no intact cells are seen. After ECM was washed, we studied the mineralization associated to these two different ECM. Matrisome proteins were identified through proteomics. DPSC matrisome is composed of 225 individual different proteins. We classified them according to different categories, the 3 core matrisome categories: glycoproteins, collagens, proteoglycans and the 3 matrisome associated proteins categories: the regulators, affiliated and secreted. When comparing the proteins in the N-ECM and OM-ECM, most of the core matrisome proteins are downregulated in OM conditions, except 3 glycoproteins, as well as regulators and secreted factors. However, annexins were found to be upregulated in OM condition. Cell adhesion, tensile strength and growth factor binding are over-represented in NM ECM. The collagen group and glycoproteins were higher in N-ECM than OM-ECM Thereafter, gingival stem cells (GSC), the less inherit osteogenic potency cells, were seeded on these N-ECM and OM-ECM. When GSCs were seeded on DPSC-derived ECM, the OM-ECM dramatically promoted mineral deposition compared with N-ECM. We hypothesize that annexins could participate in the osteogenic inductive properties. ECM plays a pivotal role in many physiological processes, it regulates cells behavior and can orient cell differentiation. Dental pulp and oral mucosa share embryological origins but differ in their reactions to insults. Dental pulp can mineralize while oral mucosa heals ad integrum. We hypothesize that ECM participate in these characteristics.

2021-09-23 | PXD018951 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data