Dataset Information

In-column thermostability of peptides

ABSTRACT: The primary objective of this study was to revise the advantages of temperature in peptide separations using state-of-the-art columns, but more importantly, to reveal possible mechanisms elevated temperature can impair proteomic bottom-up LC-MS analyses.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human) Francisella Tularensis Subsp. Holarctica (strain Lvs)

SUBMITTER: Juraj Lenco

LAB HEAD: Juraj Lenco, Ph.D.

PROVIDER: PXD019996 | Pride | 2020-11-17

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	DataDescription_02.xlsx	Xlsx
	JL703_JL697_FtularensisLVS_Digest_2uGin1uL_0l5uL_000min_01.msf	Msf
	JL703_JL697_FtularensisLVS_Digest_2uGin1uL_0l5uL_000min_01.raw	Raw
	JL703_JL697_FtularensisLVS_Digest_2uGin1uL_0l5uL_000min_02.msf	Msf
	JL703_JL697_FtularensisLVS_Digest_2uGin1uL_0l5uL_000min_02.raw	Raw

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Publications

Sense and Nonsense of Elevated Column Temperature in Proteomic Bottom-up LC-MS Analyses.

Lenčo Juraj J Šemlej Tomáš T Khalikova Maria A MA Fabrik Ivo I Švec František F

Journal of proteome research 20201021 1

Elevated column temperature represents a simple means for improving chromatographic separation of peptides. Here, we demonstrated the advantages of the column temperature in peptide separation using state-of-the-art columns. More importantly, we also determined how temperature can impair proteomic bottom-up analyses. We found that an elevated temperature in combination with the acidic pH of the mobile phase induced in-column peptide hydrolysis with high specificity to Asp and accelerated five mo ...[more]

PMID: 33085896

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