Proteomics

Dataset Information

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Reversed-phase liquid chromatography of peptides for bottom-up proteomics: A tutorial


ABSTRACT: The data were recorded for a tutorial focused on reversed-phase liquid chromatography of peptides for bottom-up proteomics. The project does not have any biological/clinical relevance.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Francisella Tularensis Subsp. Holarctica Fsc539

SUBMITTER: Juraj Lenco  

LAB HEAD: Juraj Lenco

PROVIDER: PXD036783 | Pride | 2024-08-08

REPOSITORIES: Pride

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Publications

Reversed-Phase Liquid Chromatography of Peptides for Bottom-Up Proteomics: A Tutorial.

Lenčo Juraj J   Jadeja Siddharth S   Naplekov Denis K DK   Krokhin Oleg V OV   Khalikova Maria A MA   Chocholouš Petr P   Urban Jiří J   Broeckhoven Ken K   Nováková Lucie L   Švec František F  

Journal of proteome research 20221110 12


The performance of the current bottom-up liquid chromatography hyphenated with mass spectrometry (LC-MS) analyses has undoubtedly been fueled by spectacular progress in mass spectrometry. It is thus not surprising that the MS instrument attracts the most attention during LC-MS method development, whereas optimizing conditions for peptide separation using reversed-phase liquid chromatography (RPLC) remains somewhat in its shadow. Consequently, the wisdom of the fundaments of chromatography is slo  ...[more]

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