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Reversed-phase liquid chromatography of peptides for bottom-up proteomics: A tutorial

ABSTRACT: The data were recorded for a tutorial focused on reversed-phase liquid chromatography of peptides for bottom-up proteomics. The project does not have any biological/clinical relevance.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Francisella Tularensis Subsp. Holarctica Fsc539

SUBMITTER: Juraj Lenco

LAB HEAD: Juraj Lenco

PROVIDER: PXD036783 | Pride | 2024-08-08

REPOSITORIES: Pride

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			Action	DRS
	JL987_SJ089_FTularensisLVS_Digest_1uGuL_5_Dispersion_01.raw	Raw
	JL987_SJ089_FTularensisLVS_Digest_1uGuL_5_Dispersion_02.raw	Raw
	JL987_SJ089_FTularensisLVS_Digest_1uGuL_5_Optim_01.raw	Raw
	JL987_SJ089_FTularensisLVS_Digest_1uGuL_5_Optim_02.raw	Raw
	JL987_SJ089_FTularensisLVS_Digest_1uGuL_5_ProteomeDiscoveryStudy.msf	Msf

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Publications

Reversed-Phase Liquid Chromatography of Peptides for Bottom-Up Proteomics: A Tutorial.

Lenčo Juraj J Jadeja Siddharth S Naplekov Denis K DK Krokhin Oleg V OV Khalikova Maria A MA Chocholouš Petr P Urban Jiří J Broeckhoven Ken K Nováková Lucie L Švec František F

Journal of proteome research 20221110 12

The performance of the current bottom-up liquid chromatography hyphenated with mass spectrometry (LC-MS) analyses has undoubtedly been fueled by spectacular progress in mass spectrometry. It is thus not surprising that the MS instrument attracts the most attention during LC-MS method development, whereas optimizing conditions for peptide separation using reversed-phase liquid chromatography (RPLC) remains somewhat in its shadow. Consequently, the wisdom of the fundaments of chromatography is slo ...[more]

PMID: 36355445

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The identification of bacterial protein interaction partners pointed out to new intracellular functions of Francisella tularensis glyceraldehyde-3-phosphate dehydrogenase

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