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Affinity purification of biotinylated AtACINUS and Mass Spectrometric analysis to identify PTMs on AtACINUS

ABSTRACT: AtACINUS protein is involved in regulation of alternative transcription and splicing (AS). Previously we have identified O-GlcNAcylation and phosphorylation sites on AtACINUS using LWAC and IMAC enrichment (Xu et al., 2017). To identify more modification sites, we have created AtACINUS-YFP-TurboID/acinus-2pinin transgenic lines and pulled down AtACINUS using streptavidin beads to identify post-translational modifications on AtACINUS.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Shoot

SUBMITTER: Shouling Xu

LAB HEAD: SHOULING XU

PROVIDER: PXD020749 | Pride | 2020-12-02

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	ACINUSbiotinylateddataset.xlsx	Xlsx
	Q20200303_03.pep.xml	Pepxml
	Q20200303_03.raw	Raw
	Q20200303_05.pep.xml	Pepxml
	Q20200303_05.raw	Raw

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