Proteomics

Dataset Information

0

Egg proteome in vtg1 and vtg3 knocked-out zebrafish females


ABSTRACT: CRISPR/Cas9 knock-out of the vtg1 and vtg3 genes was performed in zebrafish separately. This project involves proteomics screening for validation of the absence of targeted proteins in vtg1 and vtg3 and their variants in knocked out female zebrafish in eggs and investigating possible changes within the corresponding proteomes as a results of the absence of these specific vitellogenins and their variants.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Egg

SUBMITTER: E Com  

LAB HEAD: Charles Pineau

PROVIDER: PXD021302 | Pride | 2021-09-09

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
280_03_1E1.RAW Raw
280_03_1E1.temp.mgf Mgf
280_03_1E2.RAW Raw
280_03_1E2.temp.mgf Mgf
280_03_1E3.RAW Raw
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Publications

Knock out of specific maternal vitellogenins in zebrafish (Danio rerio) evokes vital changes in egg proteomic profiles that resemble the phenotype of poor quality eggs.

Yilmaz Ozlem O   Patinote Amelie A   Com Emmanuelle E   Pineau Charles C   Bobe Julien J  

BMC genomics 20210428 1


<h4>Background</h4>We previously reported the results of CRISPR/Cas9 knock-out (KO) of type-I and type-III vitellogenins (Vtgs) in zebrafish, which provided the first experimental evidence on essentiality and disparate functioning of Vtgs at different stages during early development. However, the specific contributions of different types of Vtg to major cellular processes remained to be investigated. The present study employed liquid chromatography and tandem mass spectrometry (LC-MS/MS) to meet  ...[more]

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