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Systematic reanalysis of co-fractionation mass spectrometry data

ABSTRACT: A total of 206 co-fractionation mass spectrometry (CF-MS) experiments were downloaded from public proteomics repositories such as PRIDE and MASSIVE, then re-analyzed with MaxQuant.

INSTRUMENT(S): Thermo Finnigan instrument model

ORGANISM(S): Homo Sapiens (human) Caenorhabditis Elegans Oryza Sativa (rice) Trypanosoma Brucei Xenopus Laevis (african Clawed Frog) Saccharomyces Cerevisiae (baker's Yeast) Zea Mays (maize) Arabidopsis Thaliana (mouse-ear Cress) Triticum Aestivum (wheat) Dictyostelium Discoideum (slime Mold) Drosophila Melanogaster (fruit Fly) Mus Musculus (mouse)

SUBMITTER: Michael Skinnider

LAB HEAD: Leonard Foster

PROVIDER: PXD022048 | Pride | 2021-04-20

REPOSITORIES: Pride

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			Action	DRS
	MaxQuantoutput.tar	Other
	raw-files.csv.zip	Csv

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Publications

Meta-analysis defines principles for the design and analysis of co-fractionation mass spectrometry experiments.

Skinnider Michael A MA Foster Leonard J LJ

Nature methods 20210701 7

Co-fractionation mass spectrometry (CF-MS) has emerged as a powerful technique for interactome mapping. However, there is little consensus on optimal strategies for the design of CF-MS experiments or their computational analysis. Here, we reanalyzed a total of 206 CF-MS experiments to generate a uniformly processed resource containing over 11 million measurements of protein abundance. We used this resource to benchmark experimental designs for CF-MS studies and systematically optimize computatio ...[more]

PMID: 34211188

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