Dataset Information

Synaptosome proteomics of tetanus neurotoxin-injected mice

ABSTRACT: Network hyperexcitability is manifested as frequent ictal discharges, often caused by unbalanced neurotransmission. We assessed synaptic changes in the hyperexcitable visual cortex of tetanus neurotoxin-injected mice (TeNT). A proteomics analysis of synaptic content revealed Carboxypeptidase E (CPE) up-regulation following TeNT injection. To quantify CPE effect on vesicle clustering, we used an ultrastructural measure of synaptic activity to investigate functional differences at excitatory and inhibitory synapses. We found homeostatic changes in hyperexcitable networks expressed as an early onset lengthening of active zones at inhibitory synapses followed by spatial reorganization at excitatory synapses. Moreover, inhibition of CPE decreases ictal discharges in vivo. This study reveals a complex landscape of homeostatic changes affecting the synaptic release machinery , differentially at inhibitory and excitatory terminals. We propose a novel homeostatic presynaptic mechanism which may impact release timing rather than synaptic strength.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Liam McDonnell

LAB HEAD: Liam McDonnell

PROVIDER: PXD022270 | Pride | 2021-09-09

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	OR1_20180710_ES803_10423051_35C_LTrap_MD_syn-TMT1_5perc.raw	Raw
	OR1_20180710_ES803_10423051_35C_LTrap_MD_syn-TMT4_Fr6.raw	Raw
	OR1_20180713_ES803_10423051_35C_LTrap_MD_syn-TMT4_Fr4.raw	Raw
	OR1_20180713_ES803_10423051_35C_LTrap_MD_syn-TMT4_Fr5.raw	Raw
	OR1_20180713_ES803_10423051_35C_LTrap_MD_syn-TMT5_Fr6.raw	Raw

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Publications

Synaptic Vesicles Dynamics in Neocortical Epilepsy.

Vannini Eleonora E Restani Laura L Dilillo Marialaura M McDonnell Liam A LA Caleo Matteo M Marra Vincenzo V

Frontiers in cellular neuroscience 20201210

Neuronal hyperexcitability often results from an unbalance between excitatory and inhibitory neurotransmission, but the synaptic alterations leading to enhanced seizure propensity are only partly understood. Taking advantage of a mouse model of neocortical epilepsy, we used a combination of photoconversion and electron microscopy to assess changes in synaptic vesicles pools <i>in vivo</i>. Our analyses reveal that epileptic networks show an early onset lengthening of active zones at inhibitory s ...[more]

PMID: 33362472

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