Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Protein quantification of mouse inner medulla (IM) sections using MS based proteomics - WT vs. Dicer(AQP2Cre+) mice

ABSTRACT: In this project we investigated the global proteome alteration of dissected mouse inner medulla (IM) sections between the WT and the Dicer(AQP2Cre+) mice. TMT labeling strategy was used to obtain protein quantification information from LC-MS based proteomic analysis.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Kidney

SUBMITTER: Lei Cheng

LAB HEAD: Robert A. Fenton

PROVIDER: PXD022327 | Pride | 2021-05-05

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	TMT_IM_FRACTION0.msf	Msf
	TMT_IM_FRACTION01_MS3.raw	Raw
	TMT_IM_FRACTION02_MS3.raw	Raw
	TMT_IM_FRACTION03_MS3.raw	Raw
	TMT_IM_FRACTION04_MS3.raw	Raw

Items per page:

1 - 5 of 10

Publications

Dysregulation of Principal Cell miRNAs Facilitates Epigenetic Regulation of AQP2 and Results in Nephrogenic Diabetes Insipidus.

Petrillo Federica F Iervolino Anna A Angrisano Tiziana T Jelen Sabina S Costanzo Vincenzo V D'Acierno Mariavittoria M Cheng Lei L Wu Qi Q Guerriero Ilaria I Mazzarella Maria Cristina MC De Falco Alfonso A D'Angelo Fulvio F Ceccarelli Michele M Caraglia Michele M Capasso Giovambattista G Fenton Robert A RA Trepiccione Francesco F

Journal of the American Society of Nephrology : JASN 20210316 6

<h4>Background</h4>MicroRNAs (miRNAs), formed by cleavage of pre-microRNA by the endoribonuclease Dicer, are critical modulators of cell function by post-transcriptionally regulating gene expression.<h4>Methods</h4>Selective ablation of Dicer in AQP2-expressing cells (Dicer<sup>AQP2Cre+</sup> mice) was used to investigate the role of miRNAs in the kidney collecting duct of mice.<h4>Results</h4>The mice had severe polyuria and nephrogenic diabetes insipidus, potentially due to greatly reduced AQP ...[more]

PMID: 33727367

Similar Datasets

Differential gene expression after ingenol mebutate (IM) treatment

Project description:We treated primary keratinocytes and SCC cells with IM for 24h to identify differentially expressed genes after treatment compared to DMSO contorl 3x DMSO keratinocytes, 3x IM 100 nM IM keratinocytes, 3x 10 uM IM keratinocytes, 3x DMSO SCC cells, 3x 1 nM IM SCC cells, 3x 100 nM IM SCC cells, 3x 10 uM IM SCC cells

2015-05-03 | E-GEOD-63308 | biostudies-arrayexpress

Expression profiling in extracellular miRNAs (plasma miRNAs) of CML patients after imatinib discontinuation (STOP-IM)

Project description:An approximately 40% of chronic myeloid leukemia (CML) patients who discontinued imatinib (IM) therapy maintained undetectable minimal residual disease (UMRD) for more than one year (STOP-IM). We set out to examine plasma miRNAs expression in CML patients who could discontinue IM, to seek the possible distinguishable biomarker in STOP-IM CML patients. We compared CML patients who sustained UMRD for more than one year after discontinuation of imatinib (STOP-IM group) with healthy volunteers (controls). In 7 patients who had discontinued IM with sustained UMRD for more than 6 months (STOP-IM group), samples were collected when IM was stopped. Seven healthy volunteers served as control. Plasma samples were harvested form 2ml of whole blood. Isolation of total RNA was performed using the mirVana PARIS kit (Ambion, Austin, TX, USA). The expression profile of miRNAs was determined using the Human Taqman miRNA Arrays A (Applied Biosystems). Synthetic ath-miR-159 (Hokkaido System Science, Hokkaido, Japan) were used as a control. QRT-PCR was carried out on an Applied Biosystems 7900HT thermal cycler using the manufacturerâ??s recommended program. With the use of SDS2.2 software and Data Assist (Thermo Fisher Sciences), the expression of plasma miRNAs was calculated based on cycle threshold (Ct) values normalized by those of ath-miR-159, which was spiked in each plasma sample. Data analysis was done using GeneSiferâ?? software (Perkin Elmer, Waltham, MA, USA). The Benjamini-Hochberg algorithm was used for estimation of false discovery rates.

2016-03-31 | E-GEOD-75391 | biostudies-arrayexpress

Comparison of gene expression profiles of wildtype and two lines of Nrf2 knockout rat livers with or without CDDO-Im treatment

Project description:Whole livers were collected from rats treated with or without CDDO-Im. Total RNA was purified using Sepazol-RNA I Super G. Gene expression was measured in livers.

2016-08-10 | E-GEOD-77377 | biostudies-arrayexpress

DICER-interacting partners in juvenile mouse testes

Project description:DICER has a well-characterized role in the processing of microRNAs (miRNAs) and small interfering RNAs (siRNA) that are important for post-transcriptional gene regulation. Emerging evidence suggests that DICER also has several non-canonical functions beyond miRNA/siRNA biogenesis, for example in transcriptional gene silencing at the chromatin level, as well as in RNA degradation and maintenance of genomic integrity. We have shown that the function of DICER in germ cells is essential for normal spermatogenesis; male mice lacking DICER in postnatal male germ cells are infertile due to severe defects in haploid differentiation. To better understand the function of DICER in male germ cells, we immunoprecipitated DICER from juvenile mouse testes and performed mass spectrometric analysis to identify DICER-interacting proteins.

2020-06-03 | PXD017772 | Pride

IM CD34+ progenitors cells

Project description:IM CD34+ vs BM CD34+ cells Keywords: parallel sample

2006-10-06 | GSE3410 | GEO

iTRAQ analysis of K562 cell line

Project description:K562 cells untreated (S) and treated (S+IM) with Imatinib as well as sensitive (S+IM) and resistant (R) to imatinib were subjected to labelled quantification by iTRAQ to identify Bcr-Abl downstream signaling components and proteins modulated in resistance respectively

2021-10-05 | PXD025173 | Pride

Phosphoproteomic analysis of liver of Dicer knockout mice

Project description:Dicer is an essential ribonuclease involved in the biogenesis of miRNA. Previous studies have reported that Dicer is dysregulated in multiple types of cancers. To investigate the downstream phosphoproteome of Dicer, we carried out phosphotyrosine profiling of the liver of Dicer knockout of mice. We employed antibody-based enrichment of phosphotyrosine containing peptides coupled with spike-in SILAC-based quantitation. Over 400 phosphoisites were identified in each condition while 290 phosphosites were quantitated in common. Amongst these, several key signaling molecules like receptor tyrosine kinase MET, MET, PDGFR, EPHA2, EPHB4 and IGF1R/INSR were hyperphosphorylated. Also, non-receptor tyrosine kinases of Src family and their downstream effector signaling partners including IRS2 and STAT3were found to be hyperphosphorylated. Our study indicates that there is significant alteration in the signaling pathways upon ablation of Dicer.

2016-07-08 | PXD000927 | Pride

Transcription profiling of Langerhans cells isolated from CD11c-Cre x Dicer wt/wt and Dicer fl/fl mice to investigate Dicer-dependent miRNA deficiency

Project description:Transcriptional profiling of Langerhans cells isolated from CD11c-Cre x Dicer wt/wt (Dicer +/+ ) and CD11c-Cre x Dicer fl/fl (Dicer-/-) mice. Langerhans cells are isolated via enzymatic digestion of skin followed by antibody staining and FACS sorting

2010-06-17 | E-MEXP-2387 | biostudies-arrayexpress

Human DICER helicase domain recruits PKR and dsRNA binding proteins during viral infection

Project description:The innate immune response against viruses mainly involves type I interferon (IFN) in mammalian cells. The exact contribution of the RNA silencing machinery remains to be established, but several recent studies indicate that the type III ribonuclease DICER can generate viral siRNAs in specific conditions. In addition, it has been proposed that type I IFN and RNA silencing could be mutually exclusive responses. In order to decipher the implication of DICER during infection of human cells with the Sindbis virus, we determined its interactome by immunoprecipitation and mass spectrometry analysis. Our results show that human DICER specifically interacts with several double-stranded RNA binding proteins and helicases during viral infection. In particular, proteins such as DHX9, ADAR-1 and the protein kinase PKR are enriched with DICER in virus-infected cells. We validated the importance of the helicase domain of DICER in its interaction with PKR and showed that it has functional consequences for the cellular response to viral infection.

2021-04-15 | PXD019093 | Pride

Expression profiling in extracellular miRNAs (plasma miRNAs) of CML patients after imatinib discontinuation (STOP-IM)

2016-03-31 | GSE75391 | GEO