Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Langerhans cells isolated from CD11c-Cre x Dicer wt/wt and Dicer fl/fl mice to investigate Dicer-dependent miRNA deficiency

ABSTRACT: Transcriptional profiling of Langerhans cells isolated from CD11c-Cre x Dicer wt/wt (Dicer +/+ ) and CD11c-Cre x Dicer fl/fl (Dicer-/-) mice. Langerhans cells are isolated via enzymatic digestion of skin followed by antibody staining and FACS sorting

ORGANISM(S): Mus musculus

SUBMITTER: Harmjan Kuipers

PROVIDER: E-MEXP-2387 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Dicer-dependent microRNAs control maturation, function, and maintenance of Langerhans cells in vivo.

Kuipers Harmjan H Schnorfeil Frauke M FM Fehling Hans-Jörg HJ Bartels Helmut H Brocker Thomas T

Journal of immunology (Baltimore, Md. : 1950) 20100607 1

Dendritic cells (DCs) are central for the induction of T cell immunity and tolerance. Fundamental for DCs to control the immune system is their differentiation from precursors into various DC subsets with distinct functions and locations in lymphoid organs and tissues. In contrast to the differentiation of epidermal Langerhans cells (LCs) and their seeding into the epidermis, LC maturation, turnover, and MHC class II Ag presentation capacities are strictly dependent on the presence of Dicer, whi ...[more]

PMID: 20530258

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Project description:Langerhans cells (LCs) are antigen presenting cells residing in the epidermis. Due to the difficulties with obtaining sufficient quantities of LCs for functional studies, many controversies exist about their origin and function. To gain insights into the molecular mechanisms underpinning LC biology and to elucidate how similar they are to a classical tissue resident DCs of myeloid origin, we undertook a whole transcriptome analysis of human skin migratory CD1a+ LCs and CD11c+ DDCs, stimulated with an epidermal pro-inflammatory cytokine, TNF-α over a time course of 24h. RNA was extracted from 250,000 human skin migratory CD1a+ LCs and CD11c+ DDCs stimulated in culture with 25ng/ml of TNF-α for 0, 2, 8 and 24h. RNA concentration and integrity was determined with an Agilent Bioanalyser (RIN of 7.0 or above) and gene expression analysis was carried out using the Human Genome U-219 Affymetrix platform by ARK-Genomics Centre, The Roslin Institute, Edinburgh. Expression data were normalised using the Robust Multichip Average (RMA) package. 2,334 transcripts regulated by exposure of skin DCs to TNF-α were identified with Bayesian Estimation of Temporal Regulation, a cut-off threshold 0.05 , for genes showing ≥ 1.5 fold difference between the maximum gene expression level and time 0 control in log2(x) RMA normalised gene expression. The interactive 3D diagram presenting the transcriptomic networks in human skin DCs can be viewed at: http://www.macrophages.com/LC_vs_DC RNA was extracted from 250,000 human skin migratory CD1a+ LCs and DDCs stimulated in culture with 25ng/ml of TNF-α for 0, 2, 8 and 24h. RNA concentration and integrity was determined with an Agilent Bioanalyser (RIN of 7.0 or above) and gene expression analysis was carried out using the Human Genome U-219 Affymetrix platform by ARK-Genomics Centre, The Roslin Institute, Edinburgh. Expression data were normalised using the Robust Multichip Average (RMA) package. 2,334 transcripts regulated by exposure of skin DCs to TNF-α were identified with Bayesian Estimation of Temporal Regulation, a cut-off threshold 0.05 , for genes showing ≥ 1.5 fold difference between the maximum gene expression level and time 0 control in log2(x) RMA normalised gene expression. *submitter cannot locate the CEL files

Dataset Information

Transcription profiling of Langerhans cells isolated from CD11c-Cre x Dicer wt/wt and Dicer fl/fl mice to investigate Dicer-dependent miRNA deficiency

Publications

Dicer-dependent microRNAs control maturation, function, and maintenance of Langerhans cells in vivo.

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