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Complexome profiling of mitochondrial complexes isolated from seedlings or mature leaves

ABSTRACT: Assembly of the plant OXPHOS complexes was studied using complexome profiling. mitochondria were extracted from seedlings or mature leaves of the reference organism Arabidopsis thaliana. In seedlings, a fast-growing tissue, mitochondria biogenesis is very active whereas in mature leaves, little biogenesis is occurring. Comparison of both datasets allow the identification of assembly intermediates that are accumulating in seedlings but not in leaves.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Etienne Meyer

LAB HEAD: Etienne H. Meyer

PROVIDER: PXD023017 | Pride | 2021-03-30

REPOSITORIES: Pride

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The assembly pathway of complex I in Arabidopsis thaliana.

Ligas Joanna J Pineau Emmanuelle E Bock Ralph R Huynen Martijn A MA Meyer Etienne H EH

The Plant journal : for cell and molecular biology 20181127 3

All present-day mitochondria originate from a single endosymbiotic event that gave rise to the last eukaryotic common ancestor more than a billion years ago. However, to date, many aspects of mitochondrial evolution have remained unresolved. Comparative genomics and proteomics have revealed a complex evolutionary origin for many mitochondrial components. To understand the evolution of the respiratory chain, we have examined both the components and the mechanisms of the assembly pathway of comple ...[more]

PMID: 30347487

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