Dataset Information

Comprehensive Analysis of Tryptic Peptides Arising from Disulfide Linkages in NISTmAb

ABSTRACT: This work presents methods for identifying and then creating a mass spectral library for disulfide-linked peptides originating from the NISTmAb, a reference material of humanized IgG1k monoclonal antibody (RM 8671). Analyses involved both partially-reduced and non-reduced samples under neutral and weakly basic conditions, followed by nanoflow liquid chromatography tandem mass spectrometry (LC-MS/MS).

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Qian Dong

LAB HEAD: Stephen E. Stein

PROVIDER: PXD023358 | Pride | 2021-02-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2014-04-03_08_IgG8670_GuanRT_TCEP_15min_LabeledPeptidesSpiked_3-24Sample_QE_ms10msms_HCD24_Res17k_115min_1ul.csv	Csv
	2014-04-03_08_IgG8670_GuanRT_TCEP_15min_LabeledPeptidesSpiked_3-24Sample_QE_ms10msms_HCD24_Res17k_115min_1ul.raw	Raw
	2014-04-03_08_IgG8670_GuanRT_TCEP_18h_LabeledPeptidesSpiked_3-24Sample_QE_ms10msms_HCD24_Res17k_1ul.csv	Csv
	2014-04-03_08_IgG8670_GuanRT_TCEP_18h_LabeledPeptidesSpiked_3-24Sample_QE_ms10msms_HCD24_Res17k_1ul.raw	Raw
	2014-04-03_08_IgG8670_GuanRT_TCEP_2h_LabeledPeptidesSpiked_3-24Sample_QE_ms10msms_HCD24_Res17k_1ul.csv	Csv

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Publications

Comprehensive Analysis of Tryptic Peptides Arising from Disulfide Linkages in NISTmAb and Their Use for Developing a Mass Spectral Library.

Dong Qian Q Yan Xinjian X Liang Yuxue Y Markey Sanford P SP Sheetlin Sergey L SL Remoroza Concepcion A CA Wallace William E WE Stein Stephen E SE

Journal of proteome research 20210208 3

This work presents methods for identifying and then creating a mass spectral library for disulfide-linked peptides originating from the NISTmAb, a reference material of the humanized IgG1k monoclonal antibody (RM 8671). Analyses involved both partially reduced and non-reduced samples under neutral and weakly basic conditions followed by nanoflow liquid chromatography tandem mass spectrometry (LC-MS/MS). Spectra of peptides containing disulfide bonds are identified by both MS1 ion and MS2 fragmen ...[more]

PMID: 33555887

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