Dataset Information

Excessive local host-graft connectivity in aged and amyloid-loaded brain

ABSTRACT: Transplantation is a clinically relevant approach for brain repair, but much remains to be understood about influences of the disease environment in the host on transplant connectivity. To explore the influence of ageing and amyloid pathology in Alzheimer's disease (AD) we examined graft connectivity using monosynaptic Rabies virus tracing in APP/PS1 mice and in 16-18 month-old wild type mice. Neurons differentiated within 4 weeks and integrated well into the host visual cortex receiving input from the regions appropriate for visual cortex. Surprisingly, however, we found a prominent several-fold increase in local visual cortex input connectivity in both amyloid-loaded and aged environment. State-of-the-art deep proteome analysis using mass spectrometry provides first insights into the composition of environments promoting or not local exuberant input connectivity. These data therefore highlight the key role of the host pathology in shaping the input connectome calling for caution in extrapolating from one to another pathological condition.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Stefanie Hauck

LAB HEAD: Stefanie M. Hauck

PROVIDER: PXD023407 | Pride | 2022-08-12

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	THJ13321X002__WT-3m_rep2_left_92-194133.raw	Raw
	THJ13321X003__WT-8m_rep1_right_92-194134.raw	Raw
	THJ13321X004__WT-17m_aged_R2_92-194135.raw	Raw
	THJ13321X005__APPPS1-8m_rep4_right_92-194136.raw	Raw
	THJ13321X006__WT-3m_intact_R4_92-194137.raw	Raw

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Excessive local host-graft connectivity in aging and amyloid-loaded brain.

Thomas Judith J Martinez-Reza Maria Fernanda MF Thorwirth Manja M Zarb Yvette Y Conzelmann Karl-Klaus KK Hauck Stefanie M SM Grade Sofia S Götz Magdalena M

Science advances 20220610 23

Transplantation is a clinically relevant approach for brain repair, but much remains to be understood about influences of the disease environment on transplant connectivity. To explore the effect of amyloid pathology in Alzheimer's disease (AD) and aging, we examined graft connectivity using monosynaptic rabies virus tracing in APP/PS1 mice and in 16- to 18-month-old wild-type (WT) mice. Transplanted neurons differentiated within 4 weeks and integrated well into the host visual cortex, receiving ...[more]

PMID: 35687689

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