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Post-translational modification mapping - CRK10 WT and crk10-A397T kinase domain

ABSTRACT: We have analysed the recombinant kinase domain of AtCRK10 (At4g23180) purified as an N-terminal His-tag from Escherichia coli cells by LC-MS/MS. Both the WT and the crk10-A39T7 mutant allele of the CRK10 kinase domain were analysed. Post-translational modifications were identified, and a MASCOT search was carried out.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Maiara Piovesana

LAB HEAD: Michaela Matthes

PROVIDER: PXD023831 | Pride | 2023-07-20

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	F006340_Mutant.dat	Other
	F006341_WT.dat	Other
	P736_Mutant.mht	Other
	P736_Mutant.raw	Raw
	P736_WT.mht	Other

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1 - 5 of 7

Publications

A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis.

Piovesana Maiara M Wood Ana K M AKM Smith Daniel P DP Deery Michael J MJ Bayliss Richard R Carrera Esther E Wellner Nikolaus N Kosik Ondrej O Napier Johnathan A JA Kurup Smita S Matthes Michaela C MC

Journal of experimental botany 20230501 10

Cysteine-rich receptor-like kinases (CRKs) are a large family of plasma membrane-bound receptors ubiquitous in higher plants. However, despite their prominence, their biological roles have remained largely elusive so far. In this study we report the characterization of an Arabidopsis mutant named crk10-A397T in which alanine 397 has been replaced by a threonine in the αC helix of the kinase domain of CRK10, known to be a crucial regulatory module in mammalian kinases. The crk10-A397T mutant is a ...[more]

PMID: 36869735

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