Proteomics

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Defining changes in the steady-state levels of ubiquitinated peptides in aging and longevity

ABSTRACT: We performed diGLY enrichment followed by label-free proteomics in C. elegans to define and quantify changes in the ubiquitinated (Ub)-proteome during the aging process. More specifically, we compared wild-type worms at the first day of adulthood with young (day 5), mid-age (day 10) and aged adults (day 15). Moreover, we assessed the Ub-modified proteome of age-matched long-lived genetic models of dietary restriction (eat-2(ad1116)) and reduced reduced insulin/IGF-1 signaling (daf-2(e1370)).

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

SUBMITTER: David Vilchez  

LAB HEAD: David Vilchez

PROVIDER: PXD024338 | Pride | 2021-05-27

REPOSITORIES: Pride

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Dataset's files

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GlyGly__K_Sites.txt Txt
N2_d10_01_20170807_Q1_AWH_ColID_398_ProjID_724_Vilchez_21.raw Raw
N2_d10_02_20170807_Q1_AWH_ColID_398_ProjID_724_Vilchez_22.raw Raw
N2_d10_03_20170809_Q1_AWH_ColID_400_ProjID_724_Vilchez_23.raw Raw
N2_d10_04_20170809_Q1_AWH_ColID_400_ProjID_724_Vilchez_24.raw Raw
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Publications

Rewiring of the ubiquitinated proteome determines ageing in C. elegans.

Koyuncu Seda S   Loureiro Rute R   Lee Hyun Ju HJ   Wagle Prerana P   Krueger Marcus M   Vilchez David D  

Nature 20210728 7871

Ageing is driven by a loss of cellular integrity<sup>1</sup>. Given the major role of ubiquitin modifications in cell function<sup>2</sup>, here we assess the link between ubiquitination and ageing by quantifying whole-proteome ubiquitin signatures in Caenorhabditis elegans. We find a remodelling of the ubiquitinated proteome during ageing, which is ameliorated by longevity paradigms such as dietary restriction and reduced insulin signalling. Notably, ageing causes a global loss of ubiquitinatio  ...[more]

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